NDLI: Magnetic Fe3S4 nanoparticles with peroxidase-like activity, and their use in a photometric enzymatic glucose assay

Content Provider	Springer Nature Link
Author	Ding, Caiping Yan, Yinghan Xiang, Dongshan Zhang, Cuiling Xian, Yuezhong
Copyright Year	2015
Abstract	Greigite magnetic nanoparticles (Fe3S4-MNPs) were prepared and reveal a peroxidase-like activity. Kinetic studies revealed a pseudo-enzymatic activity that is much higher than that of other magnetic nanomaterial-based enzyme mimetics. This finding was exploited to design a photometric enzymatic glucose assay based on the formation of H2O2 during enzymatic oxidation of glucose by glucose oxidase, and the formation of a blue product from an enzyme substrate that is catalytically oxidized by H2O2 in the presence of Fe3S4-MNPs. Glucose can be detected in the 2 to 100 μM concentration range, and the low detection limit is 0.16 μM. The method was applied to quantify glucose in human serum. In our perception, this enzyme mimetic has a large potential in that it may be used in other oxidase based assays, but also in ELISAs. Graphic Abstract Fe3S4 magnetic nanoparticles (MNPs) are shown to act as peroxidase mimetics and this was used to design a glucose oxidase (GOx) based glucose assay where the H2O2 formed during oxidation of glucose oxidizes tetramethylbenzidine (TMB) to give a blue product which can be quantified by photometry.
Starting Page	625
Ending Page	631
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	183
Issue Number	2
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2015-12-10
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Greigite nanoparticles Peroxidase mimic Hydrogen peroxide Glucose assay SEM TEM Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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