NDLI: Detection of the genomic DNA of pathogenic α-proteobacterium Ochrobactrum anthropi via magnetic DNA enrichment using pH responsive BSA@Fe3O4 nanoparticles prior to in-situ PCR and electrophoretic separation

Content Provider	Springer Nature Link
Author	Tiwari, Arpita P. Rohiwal, Sonali S. Suryavanshi, Mangesh V. Ghosh, Saral J. Pawar, Shivaji H.
Copyright Year	2015
Abstract	Magnetite nanoparticles coated with bovine serum albumin (BSA@Fe3O4 MNPs) were used for efficient isolation and detection of genomic DNA from pathogen, Ochrobactrum anthropi. The pH tunable BSA-coated magnetic nanoparticles (BSA@Fe3O4) were synthesized by co-precipitation method and are shown to be capable of adsorbing almost 99 % of calf thymus DNA, from where it also can be efficiently desorbed by changing the pH of the suspension to 8. This finding enables efficient magnetic enrichment of DNA, followed by in-situ PCR of DNA. Over all, the detection limit of the method is 0.01 pg⋅mL‾1 for the genomic DNA of Ochrobactrum anthropi. The process does not require organic solvents or sophisticated equipment. Such an approach to fabricate and employ charge responsive Fe3O4@BSA magnetic nanoparticles for detection of pathogen has potential diagnostic and clinical applications. Graphical Abstract Schematic of in-situ PCR based on DNA adsorption and magnetic enrichment by pH responsive BSA@Fe3O4 nanoparticles for sensitive pathogen detection.
Starting Page	675
Ending Page	681
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	183
Issue Number	2
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2015-12-15
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Magnetic nanoparticles DNA adsorption DNA extraction Infection Pathogen detection PCR Magnetic enrichment TEM Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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