NDLI: Electrochemistry and biosensing activity of cytochrome c immobilized on a mesoporous interface assembled from carbon nanospheres

Content Provider	Springer Nature Link
Author	Wang, Ying Bian, Xiaojun Liao, Lei Zhu, Jie Guo, Kai Kong, Jilie Liu, Baohong
Copyright Year	2012
Abstract	We report on an amperometric biosensor for hydrogen peroxide. It is obtained via layer-by-layer assembly of ordered mesoporous carbon nanospheres and poly(diallyldimethylammonium) on the surface of an indium tin oxide (ITO) glass electrode and subsequent adsorption of cytochrome c. UV–vis absorption spectroscopy was applied to characterize the process of forming the assembled layers. Cyclic voltammetry revealed a direct and quasi-reversible electron transfer between cytochrome c and the surface of the modified ITO electrode. The surface-controlled electron transfer has an apparent heterogeneous electron-transfer rate constant (k s ) of 5.9 ± 0.2 s−1 in case of the 5-layer electrode. The biosensor displays good electrocatalytic response to the reduction of H2O2, and the amperometric signal increase steadily with the concentration of H2O2 in the range from 5 μM to 1.5 mM. The detection limit is 1 μM at pH 7.4. The apparent Michaelis-Menten constant (K m ) of the sensor is 0.53 mM. We assume that the observation of a direct electron transfer of cytochrome c on mesoporous carbon nanospheres may form the basis for a feasible approach for durable and reliable detection of H2O2. Figure An amperometric biosensor for hydrogen peroxide has been fabricated via layer-by-layer assembly of mesoporous carbon nanospheres and polyelectrolyte on ITO electrode surface for the adsorption of cytochrome c. The direct electrochemistry and electrocatalytic activity of cytochrome c was achieved on the multilayer-assembled electrode, indicating a good affinity and biocompatibility of mesoporous carbon nanospheres for cytochrome c.
Starting Page	277
Ending Page	283
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	178
Issue Number	3-4
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2012-05-26
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mesoporous carbon nanospheres Layer-by-layer Cytochrome c Hydrogen peroxide Biosensor Nanotechnology Nanochemistry Analytical Chemistry Characterization and Evaluation of Materials Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Electrochemistry and biosensing activity of cytochrome c immobilized in macroporous materials

Direct electrochemistry of hemoglobin in a renewable mesoporous carbon ceramic electrode: a new kind of hydrogen peroxide biosensor

Direct electrochemistry of hemoglobin immobilized on CdS:Mn nanoparticles

Hydrogen peroxide biosensor based on the direct electrochemistry of myoglobin immobilized on ceria nanoparticles coated with multiwalled carbon nanotubesby a hydrothermal synthetic method

Hydrogen peroxide biosensor based on direct electrochemistry of hemoglobin immobilized on gold nanoparticles in a hierarchically porous zeolite

Sensitive detection of hydrogen peroxide in foodstuff using an organic–inorganic hybrid multilayer-functionalized graphene biosensing platform

Cellular biosensor based on red blood cells immobilized on Fe3O4 Core/Au Shell nanoparticles for hydrogen peroxide electroanalysis

Hydrogen peroxide biosensor based on horseradish peroxidase immobilized on chitosan-wrapped NiFe2O4 nanoparticles

Direct electrocatalytic reduction of hydrogen peroxide at a glassy carbon electrode modified with polypyrrole nanowires and platinum hollow nanospheres

Electrochemistry and biosensing activity of cytochrome c immobilized on a mesoporous interface assembled from carbon nanospheres

Similar Documents

Electrochemistry and biosensing activity of cytochrome c immobilized in macroporous materials

Direct electrochemistry of hemoglobin in a renewable mesoporous carbon ceramic electrode: a new kind of hydrogen peroxide biosensor

Direct electrochemistry of hemoglobin immobilized on CdS:Mn nanoparticles

Hydrogen peroxide biosensor based on the direct electrochemistry of myoglobin immobilized on ceria nanoparticles coated with multiwalled carbon nanotubesby a hydrothermal synthetic method

Hydrogen peroxide biosensor based on direct electrochemistry of hemoglobin immobilized on gold nanoparticles in a hierarchically porous zeolite

Sensitive detection of hydrogen peroxide in foodstuff using an organic–inorganic hybrid multilayer-functionalized graphene biosensing platform

Cellular biosensor based on red blood cells immobilized on Fe3O4 Core/Au Shell nanoparticles for hydrogen peroxide electroanalysis

Hydrogen peroxide biosensor based on horseradish peroxidase immobilized on chitosan-wrapped NiFe2O4 nanoparticles

Direct electrocatalytic reduction of hydrogen peroxide at a glassy carbon electrode modified with polypyrrole nanowires and platinum hollow nanospheres

Electrochemistry and biosensing activity of cytochrome c immobilized on a mesoporous interface assembled from carbon nanospheres