NDLI: One-step electrochemical immunosensing for simultaneous detection of two biomarkers using thionine and ferrocene as distinguishable signal tags

Content Provider	Springer Nature Link
Author	Lai, Wenqiang Zhuang, Junyang Tang, Juan Chen, Guonan Tang, Dianping
Copyright Year	2012
Abstract	We report on a new kind of electrochemical immunosensors for simultaneous determination of the biomarkers carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP). Thionine and ferrocene were applied as distinguishable electrochemical tags (and mediators) which were covalently conjugated on anti-AFP and anti-CEA antibodies, respectively, via carboxy groups. The resulting conjugates were co-immobilized on a glassy carbon electrode functionalized with gold nanoparticles. Finally, horseradish peroxidase (HRP) was immobilized onto the modified electrode. Labeled thionine and ferrocene, respectively, act as distinguishable tags for simultaneous determination of AFP and CEA due to the difference in the location of their voltammetric peaks. With a one-step immunoassay format, the analytes in the sample produced transparent immunoaffinity reaction with the corresponding antibodies on the electrode. Once the immunocomplex is formed, it partially inhibits the active center of the immobilized HRP, and this decreased the activity of HRP in terms of reduction of hydrogen peroxide. This immunosensor enables the simultaneous determination of AFP and CEA in a single run and within the same dynamic range (0.01–50 ng mL−1) and the same lower detection limit (0.01 ng mL−1). The reproducibility and stability of the immunosensors are acceptable. The dual immunosensor was applied to evaluate several specimens, and the assay results are in acceptable agreement with clinical data. Figure This contribution devises a novel multiplexed electrochemical immunoassay for simultaneous detection of alpha-fetoprotein and carcinoembryonic antigen by using thionine and ferrocene as distinguishable signal tags on a one-spot immunosensor. The assay was performed by using one-step immunoreaction between the immobilized antibodies and the analytes. Although the linear range is relatively narrow, it completely meets the requirement of clinical diagnosis.
Starting Page	357
Ending Page	365
Page Count	9
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	178
Issue Number	3-4
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2012-06-19
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Alpha-fetoprotein Carcinoembryonic antigen Electrochemical immunosensor Multiplexed immunoassay Nanotechnology Nanochemistry Analytical Chemistry Characterization and Evaluation of Materials Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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