NDLI: Electrodeposition of CdSe quantum dots and its application to an electrochemiluminescence immunoassay for α-fetoprotein

Content Provider	Springer Nature Link
Author	Peng, Shasha Zhang, Xiaoli
Copyright Year	2012
Abstract	We report on the first label-free electrochemiluminescence (ECL) immunosensor for α-fetoprotein (AFP). It is based on the use of CdSe quantum dots that were electrodeposited directly on a gold electrode from an electrolyte (containing cadmium sulfate, EDTA and selenium dioxide) by cycling the potential between 0 and -1.2 V (vs. SCE) for 60 s. The electrodeposited dots were characterized by scanning electron microscopy and energy dispersive spectroscopy. Under optimal conditions, the specific immunoreaction between AFP and anti-AFP resulted in a decrease of the ECL signal because of the steric hindrance and the transfer inhibition by peroxodisulfate. The quenching effect of the immunoreaction on the intensity of the ECL was used to establish a calibration plot which is linear in the range from 0.05 to 200 ng mL−1. The detection limit is 2 pg mL−1. The assay is highly sensitive and satisfactorily reproducible. In our opinion it opens new avenues to apply ECL in label-free biological assays. Figure We report on the first label-free electrochemiluminescence (ECL) immunosensor for α-fetoprotein (AFP). It is based on the use of CdSe quantum dots that were electrodeposited directly on a gold electrode from an electrolyte. Under optimal conditions, the specific immunoreaction between AFP and anti-AFP resulted in a decrease of the ECL signal because of the steric hindrance and the transfer inhibition by peroxodisulfate
Starting Page	323
Ending Page	330
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	178
Issue Number	3-4
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2012-06-08
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biosensor CdSe Electrochemiluminescence Electrodeposition Immunoassay Nanotechnology Nanochemistry Analytical Chemistry Characterization and Evaluation of Materials Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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