NDLI: Ultrasensitive electrochemical supersandwich DNA biosensor using a glassy carbon electrode modified with gold particle-decorated sheets of graphene oxide

Content Provider	Springer Nature Link
Author	Wang, Jiao Shi, Anqi Fang, Xian Han, Xiaowei Zhang, Yuzhong
Copyright Year	2014
Abstract	We describe a supersandwich type of electrochemical DNA biosensor based on the use of a glassy carbon electrode (GCE) modified with reduced graphene oxide (rGO) sheets that are decorated with gold nanoparticles (Au NPs). Thiolated capture DNA (probe DNA) was covalently linked to the Au NPs on the surface of the modified GCE via formation of Au-S bonds. In presence of target DNA, its 3′ terminus hybridizes with capture probe and the 5′ terminus hybridizes with signal probe labeled with Methylene Blue (MB). On increasing the concentration of target DNA, hybridization between signal probe and target DNA results in the formation of three different DNA sequences that form a supersandwich structure. The signal intensity of MB improves distinctly with increasing concentrations of target DNA in the sample solution. The assembling process on the surface of the electrode was studied by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Differential pulse voltammetry (DPV) was used to monitor the hybridization event by measuring the changes in the peak current for MB. Under optimal conditions, the peak currents in DPV for MB linearly increase with the logarithm of target DNA concentration in the range from 0.1 μM to1.0 fM, with a detection limit of 0.35 fM (at an signal/noise ratio of 3). This biosensor exhibits good selectivity, even over single-base mismatched target DNA. Figure We designed a sensitive supersandwich electrochemical DNA biosensor based on rGO sheets decorated with Au NPs. SEM and electrochemical methods were employed to investigate the assembly process of the biosensor. The biosensor exhibits high sensitivity and good specificity.
Starting Page	935
Ending Page	940
Page Count	6
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	181
Issue Number	9-10
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2014-02-13
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Supersandwich DNA biosensor Gold nanoparticles Reduced graphene oxide Differential pulse voltammetry Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Label-free electrochemical DNA biosensor based on a glassy carbon electrode modified with gold nanoparticles, polythionine, and graphene

Ultrasensitive electrochemical sensing of dopamine using reduced graphene oxide sheets decorated with p-toluenesulfonate-doped polypyrrole/Fe3O4 nanospheres

Ultrasensitive aptamer-based on-off assay for lysozyme using a glassy carbon electrode modified with gold nanoparticles and electrochemically reduced graphene oxide

Ultrasensitive direct detection of dsDNA using a glassy carbon electrode modified with thionin-functionalized multiple graphene aerogel and gold nanostars

Ultrasensitive non-enzymatic glucose sensing at near-neutral pH values via anodic stripping voltammetry using a glassy carbon electrode modified with Pt3Pd nanoparticles and reduced graphene oxide

Electrochemical gene sensor based on a glassy carbon electrode modified with hemin-functionalized reduced graphene oxide and gold nanoparticle-immobilized probe DNA

Dopamine sensor based on a glassy carbon electrode modified with a reduced graphene oxide and palladium nanoparticles composite

An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles

Electrochemical sensing of nitrite using a glassy carbon electrode modified with reduced functionalized graphene oxide decorated with flower-like zinc oxide

Ultrasensitive electrochemical supersandwich DNA biosensor using a glassy carbon electrode modified with gold particle-decorated sheets of graphene oxide

Similar Documents

Label-free electrochemical DNA biosensor based on a glassy carbon electrode modified with gold nanoparticles, polythionine, and graphene

Ultrasensitive electrochemical sensing of dopamine using reduced graphene oxide sheets decorated with p-toluenesulfonate-doped polypyrrole/Fe3O4 nanospheres

Ultrasensitive aptamer-based on-off assay for lysozyme using a glassy carbon electrode modified with gold nanoparticles and electrochemically reduced graphene oxide

Ultrasensitive direct detection of dsDNA using a glassy carbon electrode modified with thionin-functionalized multiple graphene aerogel and gold nanostars

Ultrasensitive non-enzymatic glucose sensing at near-neutral pH values via anodic stripping voltammetry using a glassy carbon electrode modified with Pt3Pd nanoparticles and reduced graphene oxide

Electrochemical gene sensor based on a glassy carbon electrode modified with hemin-functionalized reduced graphene oxide and gold nanoparticle-immobilized probe DNA

Dopamine sensor based on a glassy carbon electrode modified with a reduced graphene oxide and palladium nanoparticles composite

An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles

Electrochemical sensing of nitrite using a glassy carbon electrode modified with reduced functionalized graphene oxide decorated with flower-like zinc oxide

Ultrasensitive electrochemical supersandwich DNA biosensor using a glassy carbon electrode modified with gold particle-decorated sheets of graphene oxide