NDLI: Highly sensitive electrochemiluminescence “turn-on” aptamer sensor for lead(II) ion based on the formation of a G-quadruplex on a graphene and gold nanoparticles modified electrode

Content Provider	Springer Nature Link
Author	Hai, Hong Yang, Feng Li, Jianping
Copyright Year	2014
Abstract	We have developed a “turn on” model of an electrochemiluminescence (ECL) based assay for lead ions. It is based on the formation of a G-quadruplex from an aptamer labeled with quantum dots (QDs) and placed on an electrode modified with of graphene and gold nanoparticles (AuNPs). A hairpin capture probe was labeled with a thiol group at the 5′-end and with an amino group at the 3′-end. It was then self-assembled on the electrode modified with graphene and AuNPs. In the absence of Pb(II), the amino tag on one end of the hairpin probe is close to the surface of the electrode and therefore unable to interact with the QDs because of steric hindrance. The ECL signal is quite weak in this case. If, however, Pb(II) is added, the stem-loop of the aptamer unfolds to form a G-quadruplex. The amino group at the 3′-end will become exposed and can covalently link to a carboxy group on the surface of the CdTe QDs. This leads to strong ECL. Its intensity increases (“turns on”) with the concentration of Pb(II). Such a “turn-on” method does not suffer from the drawbacks of “turn-off” methods. ECL intensity is linearly related to the concentration of Pb(II) in the 10 p mol·L−1 to 1 n mol·L−1 range, with a 3.8 p mol·L−1 detection limit. The sensor exhibits very low detection limits, good selectivity, satisfying stability, and good repeatability. Figure A “turn on” model of ECL method was developed based on G-quadruplex of Graphene/AuNPs of aptamer probe by using quantum dots as label. ECL intensity is increased with the increase of Pb2+ concentration. The responsive ECL intensity was linearly related to the Pb2+ concentration in the range of 1.0 × 10−11 ~ 1.0 × 10−9 mol·L−1, with a detection limit of 3.82 × 10−12 mol·L−1.
Starting Page	893
Ending Page	901
Page Count	9
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	181
Issue Number	9-10
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2014-01-30
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Graphene Gold nanoparticles Aptamer Pb(II) Electrochemiluminescence Quantum dot Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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