NDLI: Aptamer biosensor for dopamine based on a gold electrode modified with carbon nanoparticles and thionine labeled gold nanoparticles as probe

Content Provider	Springer Nature Link
Author	Xu, Yaqiong Hun, Xu Liu, Fang Wen, Xiaolong Luo, Xiliang
Copyright Year	2015
Abstract	We describe a biosensor for dopamine that is based on the use of a gold electrode modified with carbon nanoparticles (CNPs) coupled to thionine labeled gold nanoparticles (AuNPs) acting as signal amplifiers. The biosensor was constructed by first modifying the CNPs on the gold electrode and adsorbing the thionine on the surface of the AuNPs, and then linking the complementary strand of the dopamine aptamer to the AuNPs via gold-thiol chemistry. Next, dopamine aptamer is added and the duplex is formed on the surface. On addition of a sample containing dopamine, it will interact with aptamer and cause the release of the electrochemical probe which then will be adsorbed on the surface of the CNP-modified gold electrode and detected by differential pulse voltammetry. The current is linearly related to the concentration of dopamine in the 30 nM to 6.0 μM ranges. The detection limit is as low as 10 nM, and the RSD is 3.1 % at a 0.3 μM level (for n = 11). The protocol was successfully applied to the determination of dopamine in spiked human urine samples. We perceive that this method holds promise as a widely applicable platform for aptamer-based electrochemical detection of small molecules. Graphical Abstract The aptamer-based sensor for dopamine is based on the use of a gold electrode modified with carbon and gold nanoparticles for signal amplification.
Starting Page	1797
Ending Page	1802
Page Count	6
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	182
Issue Number	9-10
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2015-05-10
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dopamine Carbon nanoparticles Aptamer Differential pulse voltammetry Gold nanoparticles Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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