NDLI: Surface patterning of protein matrix basement by thermal imprinting

Content Provider	Springer Nature Link
Author	Mekaru, Harutaka
Copyright Year	2016
Abstract	In order to hourly monitor the variation of specific cell growth, a grid mesh for the recording of adhesion positions on the surface of glass slides and dishes is printed. However, since the focal length of a lens, used for making observation, changes with the film thickness when using a protein matrix basement membrane, the surface of the basement membrane and the bottom glass plate cannot simultaneously be observed. In one way to address this problem, the author considered patterning fine structures directly on the surface of the protein matrix basement membrane by employing a thermal imprinting technology. As a mold, a 100-μm-wide grid and images of area-numbers were processed using laser lithography and reactive-ion-etching on a 1-inch Si wafer. For a gelatinous protein, we chose a mixture basement membrane BioCoat Matrigel, which was coated on a 35-mm-diameter dish. A protein thin layer was gelled on a dish according to the manufacturer’s specs; the dish was then placed on the bottom loading stage of our thermal nanoimprint system. After that, a Si mold was pressed against a Matrigel matrix basement by a servo motor of the thermal nanoimprint system for 30–90 s at a press force of 1 kN. And then the mold was then released from the Matrigel without cooling when a press time reached to a set value. When an imprint temperature was 75 °C, an imprinted pattern could barely be observed. On the other hand, if the mold was heated up to 100 °C or more, the imprinted grid mesh and the printed number on the Matrigel could be clearly observed. However, a heating at 125 °C seemed to have discolored white a part of the protein. Therefore, under our experimental conditions, it can be said that the optimal heating temperature exists around 100 °C. Although in the past, in conventional thermal nanoimprint lithography, a thermoplastic bearing a glass transition temperature has been targeted, the work presented here sets an example of direct patterning on biological materials such as proteins.
Starting Page	1309
Ending Page	1318
Page Count	10
File Format	PDF
ISSN	09467076
Journal	Microsystem Technologies
Volume Number	23
Issue Number	5
e-ISSN	14321858
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-01-14
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Electronics and Microelectronics, Instrumentation Nanotechnology Mechanical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Condensed Matter Physics Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering Hardware and Architecture

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Formation of metal nanostructures by high-temperature imprinting

Fast patterning microstructures using inkjet printing conformal masks

Fast patterning microstructures using inkjet printing conformal masks

Nanoparticles in polymer-matrix composites

Simulations of nanoscale thermal conduction

Development and discussion of asymmetric magnetic soft mode electromagnetic imprinting process technology

Screen-pad printing for electrode patterning on curvy surfaces

Transient thermal characterisation of hot plates

Thermal stresses on membrane based microdevices

Surface patterning of protein matrix basement by thermal imprinting

Similar Documents

Formation of metal nanostructures by high-temperature imprinting

Fast patterning microstructures using inkjet printing conformal masks

Fast patterning microstructures using inkjet printing conformal masks

Nanoparticles in polymer-matrix composites

Simulations of nanoscale thermal conduction

Development and discussion of asymmetric magnetic soft mode electromagnetic imprinting process technology

Screen-pad printing for electrode patterning on curvy surfaces

Transient thermal characterisation of hot plates

Thermal stresses on membrane based microdevices

Surface patterning of protein matrix basement by thermal imprinting