NDLI: Comparative survival analysis of 12 histidine kinase mutants of Deinococcus radiodurans after exposure to DNA-damaging agents

Content Provider	Springer Nature Link
Author	Im, Seonghun Song, Dusup Joe, Minho Kim, Dongho Park, Don Hee Lim, Sangyong
Copyright Year	2013
Abstract	Bacteria are able to adapt to changes in the environment using two-component signal transduction systems (TCSs) composed of a histidine kinase (HK) and a response regulator (RR). Deinococcus radiodurans, one of the most resistant organisms to ionizing radiation, has 20 putative HKs and 25 putative RRs. In this study, we constructed 12 D. radiodurans mutant strains lacking a gene encoding a HK and surveyed their resistance to γ-radiation, UV-B radiation (302 nm), mitomycin C (MMC), and H2O2. Five (dr0860 −, dr1174 −, dr1556 −, dr2244 −, and dr2419 −) of the 12 mutant strains showed at least a one-log cycle reduction in γ-radiation resistance. The mutations (1) dr1174, dr1227, and dr2244 and (2) dr0860, dr2416, and dr2419 caused decreases in resistance to UV radiation and MMC, respectively. Only the dr2416 and dr2419 mutant strains showed higher sensitivity to H2O2 than the wild-type. Reductions in the resistance to γ-radiation and H2O2, but not to UV and MMC, were observed in the absence of DR2415, which seems to be a cognate RR of DR2416. This result suggests that DR2415/DR2416 (DrtR/S: DNA damage response TCS) may be another TCS responsible for the extreme resistance of D. radiodurans to DNA-damaging agents.
Starting Page	781
Ending Page	789
Page Count	9
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	36
Issue Number	6
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	2013-01-25
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Deinococcus radiodurans Histidine kinase DR2416 Biotechnology Industrial and Production Engineering Environmental Engineering/Biotechnology Industrial Chemistry/Chemical Engineering Food Science
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Pigment-based whole-cell biosensor system for cadmium detection using genetically engineered Deinococcus radiodurans

Development of Cre-lox based multiple knockout system in Deinococcus radiodurans R1

Construction of DNA damage response genepprI function-deficient and function-complementary mutants inDeinococcus radiodurans

Comparative evaluation of various control schemes for fed-batch fermentation

High cell density production of Deinococcus radiodurans under optimized conditions

Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray

Electrochemical aptasensor for tetracycline detection

Understanding the bioreactor

Special issue: bioenergy

Comparative survival analysis of 12 histidine kinase mutants of Deinococcus radiodurans after exposure to DNA-damaging agents

Similar Documents

Pigment-based whole-cell biosensor system for cadmium detection using genetically engineered Deinococcus radiodurans

Development of Cre-lox based multiple knockout system in Deinococcus radiodurans R1

Construction of DNA damage response genepprI function-deficient and function-complementary mutants inDeinococcus radiodurans

Comparative evaluation of various control schemes for fed-batch fermentation

High cell density production of Deinococcus radiodurans under optimized conditions

Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray

Electrochemical aptasensor for tetracycline detection

Understanding the bioreactor

Special issue: bioenergy

Comparative survival analysis of 12 histidine kinase mutants of Deinococcus radiodurans after exposure to DNA-damaging agents