NDLI: Pigment-based whole-cell biosensor system for cadmium detection using genetically engineered Deinococcus radiodurans

Content Provider	Springer Nature Link
Author	Joe, Min Ho Lee, Kang Hee Lim, Sang Yong Im, Seong Hun Song, Hyun Pa Lee, In Soo Kim, Dong Ho
Copyright Year	2011
Abstract	In this study, a colorimetric whole-cell biosensor for cadmium (Cd) was designed using a genetically engineered red pigment producing bacterium, Deinococcus radiodurans. Based on the previous microarray data, putative promoter regions of highly Cd-inducible genes (DR_0070, DR_0659, DR_0745, and DR_2626) were screened and used for construction of lacZ reporter gene cassettes. The resultant reporter cassettes were introduced into D. radiodurans R1 to evaluate promoter activity and specificity. Among the promoters, the one derived from DR_0659 showed the highest specificity, sensitivity, and activity in response to Cd. The Cd-inducible activity was retained in the 393-bp deletion fragment (P0659-1) of the P0569 promoter, but the expression pattern of the putative promoter fragments inferred its complex regulation. The detection range was from 10 to 1 mM of Cd. The LacZ expression was increased up to 100 μM of Cd, but sharply decreased at higher concentrations. For macroscopic detection, the sensor plasmid (pRADI-P0659-1) containing crtI as a reporter gene under the control of P0659-1 was introduced into a crtI-deleted mutant strain of D. radiodurans (KDH018). The color of this sensor strain (KDH081) changed from light yellow to red by the addition of Cd and had no significant response to other metals. Color change by the red pigment synthesis could be clearly recognized in a day with the naked eye and the detection range was from 50 nM to 1 mM of Cd. These results indicate that genetically engineered D. radiodurans (KDH081) can be used to monitor the presence of Cd macroscopically.
Starting Page	265
Ending Page	272
Page Count	8
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	35
Issue Number	1-2
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-09-18
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cadmium Whole-cell biosensor Macroscopic Red pigment Deinococcus radiodurans Food Science Industrial Chemistry/Chemical Engineering Environmental Engineering/Biotechnology Industrial and Production Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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