NDLI: Proteomic analysis of Spirogyra varians mutant with high starch content and growth rate induced by gamma irradiation

Content Provider	Springer Nature Link
Author	Yoon, Minchul Choi, Jong il Kim, Gwang Hoon Kim, Dong Ho Park, Don Hee
Copyright Year	2013
Abstract	This study was conducted to develop a high-efficiency strain of Spirogyra varians for the production of biomass by radiation breeding. The characteristics of wild-type and mutant S. varians were analyzed through phenomenological and proteomic observations. The results of our phenomenological observations of the S. varians mutant demonstrated increases in growth rate and content of chlorophyll a, b, and a + b; in particular, a significant threefold increase was observed in starch accumulation. Proteomic analysis to investigate the differences in expression between wild-type and mutant proteins identified 18 proteins with significantly different expressions. From the literature review, it was confirmed that the up-regulated proteins were mainly involved in photosynthesis, carbohydrate biosynthesis, and energy metabolism. These results suggest the possibility of algae development by radiation breeding for the production of biofuel.
Starting Page	765
Ending Page	774
Page Count	10
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	36
Issue Number	6
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	2013-02-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Spirogyra varians Gamma irradiation Mutant Starch Proteome Biofuel Biotechnology Industrial and Production Engineering Environmental Engineering/Biotechnology Industrial Chemistry/Chemical Engineering Food Science
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

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