NDLI: Analysis of the expression of cytoskeletal proteins of Taenia crassiceps ORF strain cysticerci (Cestoda)

Content Provider	Springer Nature Link
Author	Reyso Ducoing, Olivia Valverde Islas, Laura Paredes Salomon, Cristina Pérez Reyes, América Landa, Abraham Robert, Lilia Mendoza, Guillermo Ambrosio, Javier R.
Copyright Year	2014
Abstract	The Taenia crassiceps ORF strain is used to generate a murine model of cysticercosis, which is used for diagnosis, evaluation of drugs, and vaccination. This particular strain only exists as cysticerci, is easily maintained under in vivo and in vitro conditions, and offers an excellent model for studying the cytoskeletons of cestodes. In this study, several experimental approaches were used to determine the tissue expression of its cytoskeletal proteins. The techniques used were microscopy (video, confocal, and transmission electron), one-dimensional (1D) and two-dimensional (2D) electrophoresis, immunochemistry, and mass spectrometry. The tissue expression of actin, tubulin, and paramyosin was assessed using microscopy, and their protein isoforms were determined with 1D and 2D electrophoresis and immunochemistry. Nineteen spots were excised from a proteomic gel and identified by liquid chromatography–tandem mass spectrometry and immunochemistry. The proteins identified were classic cytoskeletal proteins, metabolic enzymes, and proteins with diverse biological functions, but mainly involved in detoxification activities. Research suggests that most noncytoskeletal proteins interact with actin or tubulin, and the results of the present study suggest that the proteins identified may be involved in supporting the dynamics and plasticity of the cytoskeleton of T. crassiceps cysticerci. These results contribute to our knowledge of the cellular biology and physiology of cestodes.
Starting Page	1955
Ending Page	1969
Page Count	15
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	113
Issue Number	5
e-ISSN	14321955
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-03-21
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Taenia crassiceps Cysticerci Cytoskeleton Proteomics Immunohistochemistry Protein isoforms Medical Microbiology Microbiology Immunology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Differential Protein Expression of Taenia crassiceps ORF Strain in the Murine Cysticercosis Model Using Resistant (C57BL/6) Mice.

In vivo albendazole treatment of Taenia crassiceps cysticerci strain WFU: proliferation, damage, and recovery

Glucose 6-phosphate dehydrogenase from larval Taenia crassiceps (cysticerci): purification and properties

RCB20, an experimental benzimidazole derivative, affects tubulin expression and induces gross anatomical changes in Taenia crassiceps cysticerci

In vitro killing action of auranofin on Taenia crassiceps metacestode (cysticerci) and inactivation of thioredoxin–glutathione reductase (TGR)

Metabolism of [3-13C]-pyruvate by cysticerci of Taenia crassiceps

Characterization of one typical 2-Cys Peroxiredoxin gene of Taenia solium and Taenia crassiceps

Taenia solium cysticerci synthesize androgens and estrogens in vitro

Ultrastructure of a spermatid transport system in the mature proglottids of experimental Taenia crassiceps (WFU strain)

Analysis of the expression of cytoskeletal proteins of Taenia crassiceps ORF strain cysticerci (Cestoda)

Similar Documents

Differential Protein Expression of Taenia crassiceps ORF Strain in the Murine Cysticercosis Model Using Resistant (C57BL/6) Mice.

In vivo albendazole treatment of Taenia crassiceps cysticerci strain WFU: proliferation, damage, and recovery

Glucose 6-phosphate dehydrogenase from larval Taenia crassiceps (cysticerci): purification and properties

RCB20, an experimental benzimidazole derivative, affects tubulin expression and induces gross anatomical changes in Taenia crassiceps cysticerci

In vitro killing action of auranofin on Taenia crassiceps metacestode (cysticerci) and inactivation of thioredoxin–glutathione reductase (TGR)

Metabolism of [3-13C]-pyruvate by cysticerci of Taenia crassiceps

Characterization of one typical 2-Cys Peroxiredoxin gene of Taenia solium and Taenia crassiceps

Taenia solium cysticerci synthesize androgens and estrogens in vitro

Ultrastructure of a spermatid transport system in the mature proglottids of experimental Taenia crassiceps (WFU strain)

Analysis of the expression of cytoskeletal proteins of Taenia crassiceps ORF strain cysticerci (Cestoda)