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Planta : Volume 216, Issue 3, January 2003

Planta : Volume 216, Issue 2, December 2002

Planta : Volume 216, Issue 1, November 2002

Expression and localization of human lysozyme in the endosperm of transgenic rice

Content Provider	Springer Nature Link
Author	Yang, Daichang Guo, Fengli Liu, Bo Huang, Ning Watkins, Simon C.
Copyright Year	2003
Abstract	In order to understand the characteristics of recombinant protein expression and sublocalization in rice (Oryza sativa L.) endosperm, we examined the expression level of human lysozyme protein and its subcellular location in transgenic rice seeds driven by rice glutelin and globulin promoters and signal peptides. A time course of human lysozyme expression during endosperm development was analyzed. The results showed that the expression profile of recombinant protein accumulation in endosperm paralleled that of the two storage proteins. Immunofluorescence microscopy revealed that human lysozyme and storage proteins co-localized to type-II protein bodies. Both promoter–signal peptide parings targeted recombinant protein to the protein bodies. In addition, a transgenic line with a higher lysozyme expression level exhibited morphologically different protein bodies with an unbalanced composition of lysozyme and native storage proteins. The high-level expression of recombinant protein distorted the trafficking and sorting of native storage proteins in rice endosperm and affected the expression of native storage protein.
Starting Page	597
Ending Page	603
Page Count	7
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	216
Issue Number	4
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-10-17
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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