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Planta : Volume 216, Issue 3, January 2003

Planta : Volume 216, Issue 2, December 2002

Planta : Volume 216, Issue 1, November 2002

Glutamate synthesis in barley roots: the role of the plastidic glucose-6-phosphate dehydrogenase

Content Provider	Springer Nature Link
Author	Esposito, Sergio Massaro, Graziella Vona, Vincenza Di Marti Riga, Vittoria Carfagna, Simona
Copyright Year	2003
Abstract	Evidence is provided for a close link between glutamate (Glu) synthesis and the production of reducing power by the oxidative pentose phosphate pathway (OPPP) in barley (Hordeum vulgare L. var. Alfeo) root plastids. A rapid procedure for isolating organelles gave yields of plastids of over 30%, 60% of which were intact. The formation of Glu by intact plastids fed with glutamine and 2-oxoglutarate, both substrates of glutamate synthase (GOGAT), depends on glucose-6-phosphate (Glc-6-P) supply. The whole process exhibited an apparent K m Glc-6-P of 0.45 mM and is abolished by azaserine, a specific inhibitor of GOGAT; ATP caused a decrease in the rate of Glu formation. Glucose and other sugar phosphates were not as effective in supporting Glu synthesis with respect to Glc-6-P; only ribose-5-phosphate, an intermediate of OPPP, supported rates equivalent to Glc-6-P. Glucose-6-phosphate dehydrogenase (Glc6PDH) rapidly purified from root plastids showed an apparent K m Glc-6-P of 0.96 mM and an apparent K m NADP + of 9 µM. The enzyme demonstrated high tolerance to NADPH, exhibiting a K i NADPH of 58.6 µM and selectively reacted with antibodies against potato plastidic, but not chloroplastic, Glc6PDH isoform. The data support the hypothesis that plastidic OPPP is the main site of reducing power supply for GOGAT within the plastids, and suggest that the plastidic OPPP would be able to sustain Glu synthesis under high NADPH:NADP+ ratios even if the plastidic Glc6PDH may not be functioning at its highest rates.
Starting Page	639
Ending Page	647
Page Count	9
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	216
Issue Number	4
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-09-13
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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