NDLI: MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana

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Planta : Volume 216, Issue 3, January 2003

Planta : Volume 216, Issue 2, December 2002

Planta : Volume 216, Issue 1, November 2002

MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana

Content Provider	Springer Nature Link
Author	Chakrabarti, A. Ganapathi, T. R. Mukherjee, P. K. Bapat, V. A.
Copyright Year	2003
Abstract	Magainin is one of the earliest reported antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis. A synthetic substitution analogue of magainin, MSI-99, is employed in this study to impart disease resistance in transgenic tobacco ( Nicotiana tabacum L.) and banana [( Musa spp. cv. Rasthali (AAB)]. This peptide inhibited the growth and spore germination of Fusarium oxysporum f.sp. cubense at 16 µg/ml. MSI-99 has been subcloned into plant expression vectors pMSI164 and pMSI168, targeting the peptide into the cytoplasm and extracellular spaces, respectively. Tobacco plants transformed with pMSI168 showed enhanced resistance against Sclerotinia sclerotiorum, Alternaria alternata and Botrytis cinerea. Transgenic banana pants were obtained for both pMSI164 and pMSI168 transformations and showed resistance to F. oxysporum f.sp. cubense and Mycosphaerella musicola. The transgenic nature of the transformants and expression of this peptide was confirmed through polymerase chain reaction (PCR) and reverse transcription (RT)–PCR. The results suggest that MSI-99 can be useful in imparting enhanced disease resistance in transgenic plants.
Starting Page	587
Ending Page	596
Page Count	10
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	216
Issue Number	4
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-12-06
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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