NDLI: Histological analysis of direct somatic embryogenesis in Arabidopsis thaliana (L.) Heynh

Content Provider	Springer Nature Link
Author	Kurczyńska, Ewa U. Gaj, Małgorzata D. Ujczak, Agnieszka Mazur, Ewa
Copyright Year	2007
Abstract	In Arabidopsis the in vitro culture of immature zygotic embryos (IZEs) at a late stage of development, on the solid medium containing synthetic auxin, leads to formation of somatic embryos via direct somatic embryogenesis (DSE). The presented results provide evidence that in IZE cells competent for DSE are located in the protodermis and subprotodermis of the adaxial side of cotyledons and somatic embryos displayed a single- or multicellular origin. Transgenic Arabidopsis lines expressing the GUS reporter gene, driven by the DR5 and LEC2 promoters, were used to analyse the distribution of auxin to mark embryogenic cells in cultured explants and develop somatic embryos. The analysis showed that at the start of the culture auxin was accumulated in all explant tissues, but from the fourth day onwards its location shifted to the protodermis and subprotodermis of the explant cotyledons. In globular somatic embryos auxin was detected in all cells, with a higher concentration in the protodermis, and in the heart stage its activity was mainly displayed in the shoot, root pole and cotyledon primordia. The embryogenic nature of dividing protodermal and subprotodermal cells accumulating auxin was confirmed by high expression of promoter activity of LEC2 in these cells. Analysis of symplasmic tracer (CFDA) distribution indicated symplasmic isolation between tissues engaged in DSE and other parts of an explant. Symplasmic isolation of somatic embryos from the explant was also detected.
Starting Page	619
Ending Page	628
Page Count	10
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	226
Issue Number	3
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-04-04
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arabidopsis Auxin Direct somatic embryogenesis DR5::GUS construct LEC2 gene Symplasmic communication Forestry Ecology Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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