NDLI: Characterisation of a prokaryote-type tRNA-isopentenyltransferase gene from the moss Physcomitrella patens

Content Provider	Springer Nature Link
Author	Yevdakova, Natalya A. Schwartzenberg, Klaus
Copyright Year	2007
Abstract	Cytokinins are of critical importance to numerous developmental processes in plants. Two cytokinin biosynthetic pathways have been described; each one uses a different type of isopentenyltransferases (IPTs) as the key enzyme. In the first pathway, adenylate-IPTs (EC 2.5.1.27) prenylate adenylic nucleotides to cytokinin nucleotides, thus catalysing the direct de novo biosynthesis of free cytokinins. In the second pathway, tRNA-IPTs (EC 2.5.1.8) catalyse cytokinin formation by isopentenylation of tRNA, the degradation of which liberates cytokinin nucleotides. Seed plants have been shown to possess both forms of IPTs. Here, we report on the in-silico based identification and on the functional characterisation of an IPT encoding gene (PpIPT1) from the bryophyte Physcomitrella patens. Analysis of the PpIPT1 amino acid sequence revealed high similarities to tRNA-IPTs of other plants. No adenylate-IPT genes were found in the Physcomitrella sequenced transcriptome/genome. PpIPT1 functionally complemented a defective tRNA-IPT gene of Saccharomyces cerevisiae (ScMOD5) in the strain MT-8. Dephosphorylated tRNA hydrolysates from PpIPT1-transformed MT-8 showed cytokinin activity in a moss bioassay and the presence of isopentenyladenosine in HPLC analysis, in contrast to those prepared from untransformed MT-8. A comparison of pro- and eukaryotic homologues revealed two classes of tRNA-IPTs; PpIPT1 belongs to a prokaryotic type with predicted chloroplast targeting. RT-PCR experiments revealed a stronger expression in the cytokinin overproducing mutant oveST25, thus indicating the potential role of PpIPT1 for cytokinin biosynthesis in the evolutionary old land plant Physcomitrella.
Starting Page	683
Ending Page	695
Page Count	13
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	226
Issue Number	3
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-04-21
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cytokinin biosynthesis Isopentenyltransferase tRNA Forestry Ecology Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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Identification of two additional members of the tRNA isopentenyltransferase family in Physcomitrella patens

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