NDLI: Leafy cotyledon genes are essential for induction of somatic embryogenesis of Arabidopsis

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Leafy cotyledon genes are essential for induction of somatic embryogenesis of Arabidopsis

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Planta : Volume 222, Issue 4, November 2005

Planta : Volume 222, Issue 5, November 2005

Planta : Volume 222, Issue 2, October 2005

Planta : Volume 222, Issue 3, October 2005

Planta : Volume 222, Issue 1, September 2005

Leafy cotyledon genes are essential for induction of somatic embryogenesis of Arabidopsis

Content Provider	Springer Nature Link
Author	Gaj, Malgorzata D. Zhang, Shibo Harada, John J. Lemaux, Peggy G.
Copyright Year	2005
Abstract	The capacity for somatic embryogenesis was studied in lec1, lec2 and fus3 mutants of Arabidopsis thaliana (L.) Heynh. It was found that contrary to the response of wild-type cultures, which produced somatic embryos via an efficient, direct process (65–94% of responding explants), lec mutants were strongly impaired in their embryogenic response. Cultures of the mutants formed somatic embryos at a low frequency, ranging from 0.0 to 3.9%. Moreover, somatic embryos were formed from callus tissue through an indirect route in the lec mutants. Total repression of embryogenic potential was observed in double (lec1 lec2, lec1 fus3, lec2 fus3) and triple (fus3 lec1 lec2) mutants. Additionally, mutants were found to exhibit efficient shoot regenerability via organogenesis from root explants. These results provide evidence that, besides their key role in controlling many different aspects of Arabidopsis zygotic embryogenesis, LEC/FUS genes are also essential for in vitro somatic embryogenesis induction. Furthermore, temporal and spatial patterns of auxin distribution during somatic embryogenesis induction were analyzed using transgenic Arabidopsis plants expressing GUS driven by the DR5 promoter. Analysis of data indicated auxin accumulation was rapid in all tissues of the explants of both wild type and the lec2-1 mutant, cultured on somatic embryogenesis induction medium containing 2,4-D. This observation suggests that loss of embryogenic potential in the lec2 mutant in vitro is not related to the distribution of exogenously applied auxin and LEC genes likely function downstream in auxin-induced somatic embryogenesis.
Starting Page	977
Ending Page	988
Page Count	12
File Format	PDF
ISSN	00320935
Journal	Planta
Volume Number	222
Issue Number	6
e-ISSN	14322048
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-07-21
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arabidopsis Auxin Immature zygotic embryo culture LEC/FUS genes lec/fus mutants Somatic embryogenesis Forestry Ecology Agriculture Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science

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