NDLI: Voltage-dependent ion channel currents in putative neuroendocrine cells dissociated from the ventral prostate of rat

Content Provider	Springer Nature Link
Author	Kim, Jun Hee Shin, Sun Young Yun, Sang Soon Kim, Tae Jin Oh, Seung June Kim, Kwang Myung Chung, Young Shin Hong, Eun Kyoung Uhm, Dae Yong Kim, Sung Joon
Copyright Year	2003
Abstract	Prostate neuroendocrine (NE) cells play important roles in the growth and differentiation of the prostate. Following enzymatic digestion of rat ventral prostate, the whole-cell patch-clamp technique was applied to dark, round cells that exhibited chromogranin-A immunoreactivity, a representative marker of NE cells. Under zero current-clamp conditions, putative NE cells showed hyperpolarized resting membrane potentials of some −70 mV, and spontaneous action potentials were induced by an increase in external [K+] or by the injection of current. Using a CsCl pipette solution, step-like depolarization activated high-voltage-activated Ca2+ current (HVA I Ca) and tetrodotoxin-resistant voltage-activated Na+ current. The HVA I Ca was blocked by nifedipine and ω-conotoxin GVIA, L-type and N-type Ca2+ channel blockers, respectively. Using a KCl pipette solution, the transient outward K+ current (I to), Ca2+-activated K+ currents (I K,Ca), the non-inactivating outward current and an inwardly rectifying K+ current (I Kir) were identified. I K,Ca was suppressed by charybdotoxin (50 nM), iberiotoxin (10 nM) or clotrimazol (1 μM), but not by apamine (100 nM). I to was inhibited by 4-aminopyridine (5 mM). I Kir was identified as a Ba2+-sensitive inwardly rectifying current in the presence of a high-K+ bath solution. The voltage- and Ca2+-activated ion channels could play significant roles in the regulation of neurohormonal secretion in the prostate.
Starting Page	88
Ending Page	99
Page Count	12
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	446
Issue Number	1
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-02-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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