NDLI: Pharmacological investigation of the role of ion channels in salivary secretion

Content Provider	Springer Nature Link
Author	Stummann, Tina C. Poulsen, Jørgen H. Hay Schmidt, Anders Grunnet, Morten Klaerke, Dan A. Rasmussen, Hanne B. Olesen, Søren Peter Jorgensen, Nanna K.
Copyright Year	2003
Abstract	The role of K+ and Cl− channels in salivary secretion was investigated, with emphasis on the potential role of Ca2+-activated K+ channels. Ligand saturation kinetic assays and autoradiography showed large-conductance (BK) K+ channels to be highly expressed in rat submandibular and parotid glands, whereas low-conductance (SK) K+ channels could not be detected. To investigate the role of K+ and Cl− channels in secretion, intact rabbit submandibular glands were vascularly perfused and secretion induced by 10 µM ACh. Secretion was inhibited by 34±3% following perfusion with the general K+ channel inhibitor Ba2+ (5 mM), whereas organic inhibitors of BK (200 nM paxilline) or intermediate-conductance (IK) K+ channels (5 µM clotrimazole) had no effect. Secretion was strongly influenced by Cl− channel inhibitors, as 100 µM 5-nitro-2-(3-phenylpropylamino)benzoate (NPPB) completely abolished, while 10 µM NPPB, 20 µM NS1652 and 20 µM NS3623 reduced secretion by 34±3%, 23±3% and 59±4%, respectively. In conclusion, although high expression levels of BK channels were demonstrated, pharmacological tools failed to demonstrate any role for BK, IK or SK channels in salivary secretion in the rabbit submandibular gland. Other types of K+ channel, however, and particularly Cl− channels, are essential for ACh-induced salivary secretion.
Starting Page	78
Ending Page	87
Page Count	10
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	446
Issue Number	1
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-02-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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