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Voltage-dependent calcium currents in dissociated granule cells from rat cerebellum
| Content Provider | Semantic Scholar |
|---|---|
| Author | Marchetti, Cristina Carignani, Corrado Robello, Mauro |
| Copyright Year | 1991 |
| Abstract | Voltage-dependent calcium currents were investigated by the patch-clamp technique in whole-cell recording configuration in cultures from 8-day-old rat cerebella, which contained greater than or equal to 90% granule cells. In solutions designed to minimize the sodium and potassium conductances and in 20 mM barium, an inward current activated around -25 mV, reached a peak amplitude at +20 mV and reversed around +80 mV. In 20 mM calcium, this current was approximately 50% of that in barium. From one to three days in vitro only 16% of the cells tested (n = 20) had a current exceeding 50 pA in maximum amplitude, while after four days in vitro the current reached 100 pA in all neurons tested (n greater than 70). Verapamil (50-100 microM) reversibly depressed this current. The dihydropyridine agonist Bay K 8644 (1 microM) enhanced the maximum conductance by 25 +/- 8% (n = 4), caused a negative shift in the activation of 21 +/- 5 mV and a prolongation of the deactivation time course as the voltage was stepped back from +20 to -80 mV. The GABAB agonist baclofen (50 microM) reversibly depressed the current by 27 +/- 8% in 80% of the cells. The effect was similar to that of GABA (10 microM), when the GABAA response (chloride current) was partially blocked by bicucculine. This current can be classified as a dihydropyridine-sensitive high-voltage-activated calcium current. The modulation by GABAB agonists is likely to be significant for presynaptic inhibition. |
| Starting Page | 121 |
| Ending Page | 133 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0306-4522(91)90422-K |
| PubMed reference number | 1717882 |
| Journal | Medline |
| Volume Number | 43 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/030645229190422K |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/030645229190422K?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0306-4522%2891%2990422-K |
| Journal | Neuroscience |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
