NDLI: Properties of T-type calcium current in enkephalinergic neurones in guinea-pig hypothalamic slices

Content Provider	Springer Nature Link
Author	Niespodziany, Isabelle Derambure, Philippe Poulain, P.
Copyright Year	1999
Abstract	The guinea-pig hypothalamic magnocellular dorsal nucleus (mdn) exclusively contains enkephalinergic neurones providing inputs to the septum. This nucleus is believed to play a role in hippocampo-septo-hypothalamic relationships. mdn neurones display prominent low-threshold Ca2+ spikes, which differ in their propensity to trigger either a burst of Na+ spikes or a single spike. In the present study, whole-cell voltage-clamp experiments were carried out on thick slices at 34°C to characterize the pharmacological and physical properties of the transient Ca2+ current (I T) underlying the low-threshold spikes. Recorded cells were dye-labelled and identified as belonging to the mdn. In bursting and non-bursting neurones, I T was reduced by amiloride (1 µM) and octanol (1 mM), and during replacement of Ca2+ by Ba2+. The Ca2+ channel blocker mibefradil (10 µM) had only a slight blocking action. Nifedipine (100 µM) and flunarizine (1 µM) had no effect. I T activated between –80 mV and –50 mV and the mean peak current was 1050 pA. Steady-state activation and inactivation curves were fitted by a Boltzmann equation. The half-activation voltage was –70 mV, slope factor=3.6, and half-inactivation voltage was about –80 mV, slope factor=4.5. Time-to-peak and time constant of inactivation were voltage dependent. Recovery from activation occurred within 500 ms. When compared with results on other I T, the present data show that the current possesses distinct pharmacological and physical properties. Nevertheless, all investigated cells displayed a homogenous profile of I T, suggesting that the differences in spike pattern between mdn neurones are not due to different populations of Ca2+ channels.
Starting Page	871
Ending Page	880
Page Count	10
File Format	PDF
ISSN	00316768
Journal	Pflügers Archiv
Volume Number	437
Issue Number	6
e-ISSN	14322013
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-04-22
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Clinical Biochemistry

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