NDLI: Oestrogen receptor β is present in both muscle fibres and endothelial cells within human skeletal muscle tissue

Content Provider	Springer Nature Link
Author	Wiik, Anna Ekman, Marianne Morgan, Gareth Johansson, Olle Jansson, Eva Esbjörnsson, Mona
Copyright Year	2005
Abstract	Oestrogen receptor β (ERβ) is expressed in human skeletal muscle tissue. In the present study, we have developed an immunohistochemical method to reveal if ERβ is located within the muscle fibres as well as within capillaries. Skeletal muscle biopsies were obtained from m. quadriceps femoris vastus lateralis in four healthy young subjects. Immunohistochemical triple staining was applied to transverse sections of paraffin-wax-embedded tissue. The basement membrane of muscle fibres and capillaries was identified by using an antibody to collagen IV, endothelial cells using an antibody to CD34 and ERβ using a corresponding antibody. The ERβ-positive (ERβ+) nuclei were located within the muscle fibre defined by the localisation of collagen IV. ERβ+ nuclei were also, for the first time, found in endothelial cells of capillaries in skeletal muscle tissue. Quantification was performed on transverse cryostat sections after performing a double staining (collagen IV and ERβ). It was shown that 24% of the ERβ+ nuclei were located within capillaries, and 76% were located within muscle fibres. In conclusion, ERβ in human skeletal muscle tissue is expressed not only in the muscle fibres themselves, but also within the capillary endothelial cells. This observation might improve understanding of the physiological role of oestrogen and its receptor.
Starting Page	161
Ending Page	165
Page Count	5
File Format	PDF
ISSN	09486143
Journal	Histochemistry and Cell Biology
Volume Number	124
Issue Number	2
e-ISSN	1432119X
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-08-13
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	capillaries CD34 collagen IV immunohistochemistry triple staining Medicine/Public Health Anatomy
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Molecular Biology Medical Laboratory Technology

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