NDLI: Relative Expression of Low Molecular Weight Protein, Tyrosine Phosphatase (Wzb Gene) of Herbaspirillum sp. GW103 Toward Arsenic Stress and Molecular Modeling

Content Provider	Springer Nature Link
Author	Govarthanan, Muthusamy Park, Jung Hee Praburaman, Loganathan Yi, Young Joo Cho, Min Myung, Hyun Gnanendra, Shanmugam Kamala Kannan, Seralathan Oh, Byung Taek
Copyright Year	2015
Abstract	This study investigated the expression rate and molecular modeling of Wzb gene, a low molecular weight protein tyrosine phosphatase, under As stress in Herbaspirillum sp. GW103. Expression of Wzb gene was quantified at transcriptional level through real-time quantitative PCR. The results showed up- and down-regulations of Wzb gene in the presence of As (50 and 100 mg/L). The maximum Wzb transcript expression was 1.2-fold after 72 h exposure to 50 mg/L of As. However, the minimum expression was 0.1-fold after 48 h exposure to 100 mg/L of As. The Wzb protein sequence was retrieved from NCBI sequence database and was used for in silico analysis. 3D structure of Wzb gene was predicted by comparative modeling using modeler 9v9. Further, the model was validated for its quality by Ramachandran plot, ERRAT, Verify 3D, and SAVES server which revealed structure and quality of the Wzb gene model.
Starting Page	311
Ending Page	316
Page Count	6
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	71
Issue Number	3
e-ISSN	14320991
Language	English
Publisher	Springer US
Publisher Date	2015-06-06
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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