NDLI: Cellular Proteins Act as Bridge Between 5′ and 3′ Ends of the Coxsackievirus B3 Mediating Genome Circularization During RNA Translation

Content Provider	Springer Nature Link
Author	Souii, Amira M’hadheb Gharbi, Manel Ben Gharbi, Jawhar
Copyright Year	2015
Abstract	The positive single-stranded RNA genome of the Coxsackievirus B3 (CVB3) contains a 5′ untranslated region (UTR) which hosts the internal ribosome entry site (IRES) element that governs cap-independent translation initiation and a polyadenylated 3′ UTR which is required for stimulating the IRES activity. Viral RNA genomes could circularize to regulate initiation of translation and RNA synthesis at 5′ and 3′ ends. Interactions could either take place by direct RNA–RNA contacts, through cellular protein bridges mediating RNA circularization or both. Accordingly, we aimed to assess the nature of molecular interactions between these two regions and to evaluate cellular factors required for mRNA 3′ end-mediated stimulation of CVB3 IRES-driven translation. By gel shift assays, we have showed that combining, in vitro, 5′ and 3′ UTR fragments had no discernible effect on the structures of RNAs, arguing against the presence of specific canonical RNA–RNA cyclization sequences between these two regions. Competitive UV crosslinking assays using BHK-21 cell extract showed common cellular proteins eIF3b, PTB, and La binding to both 5′- and 3′ end RNAs. PCBP 1–2 and PABP were shown to bind, respectively, to 5′ and 3′ UTR probes. Taking together, these data suggest that CVB3 5′–3′ end bridging occurs through 5′ UTR–protein–protein–3′ UTR interactions and not through RNA–RNA direct contact. The dual involvement of the 3′ and 5′ UTRs in controlling viral translation and RNA synthesis highlights the relevance of these regions in the infectious virus life cycle, making them suitable candidates for targeted CVB3 antiviral therapy.
Starting Page	387
Ending Page	395
Page Count	9
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	71
Issue Number	3
e-ISSN	14320991
Language	English
Publisher	Springer US
Publisher Date	2015-07-03
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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