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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Loganathan, Praburaman Myung, Hyun Muthusamy, Govarthanan Lee, Kui-Jae Seralathan, Kamala-Kannan Oh, Byung-Taek |
| Description | Country affiliation: South Korea Author Affiliation: Loganathan P ( Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.); Myung H ( Department of Ecology Landscape Architecture-Design, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.); Muthusamy G ( Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.); Lee KJ ( Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.); Seralathan KK ( Plant Medical Research Center, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Jeonbuk, South Korea.); Oh BT ( Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea.) |
| Abstract | This study aimed to understand the influence of heavy metals on 1-aminocyclopropane-1-carboxylate deaminase activity (ACCD) and acdS gene expression in Herbaspirillium sp. GW103. The GW103 strain ACCD activity decreased in cells grown in a medium supplemented with Pb and As, whereas cells grown in medium supplemented with Cu showed increase in enzyme activity. The GW103 strain produced 262.2 ± 6.17 µmol of -ketobutyrate per milligram of protein per hour during ACC deamination at 25 °C after 24 h incubation. Using a PCR approach, an acdS coding-gene of 1.06 kbp was amplified in isolate GW103, showing 92% identity with Herbaspirillum seropedicae SmR1 acdS gene. Real time quantitative polymerase chain reaction results indicate that the acdS expression rate was increased (7.1-fold) in the presence of Cu, whereas it decreased (0.2- and 0.1-fold) in the presence of As and Pb. |
| File Format | HTM / HTML |
| ISSN | 0233111X |
| Issue Number | 10 |
| Volume Number | 55 |
| e-ISSN | 15214028 |
| Journal | Journal of Basic Microbiology |
| Language | English |
| Publisher | Wiley-VCH Verlag |
| Publisher Date | 2015-10-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Gene Expression Regulation, Bacterial Genes, Bacterial Herbaspirillum Enzymology Genetics Metals, Heavy Metabolism Soil Microbiology Arsenic Carbon-carbon Lyases Copper Dna, Bacterial Isolation & Purification Lead Plant Roots Real-time Polymerase Chain Reaction Rhizosphere Sequence Analysis, Dna Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Applied Microbiology and Biotechnology |
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