NDLI: Isolation and Comparative Genomic Analysis of T1-Like Shigella Bacteriophage pSf-2

Content Provider	Springer Nature Link
Author	Jun, Jin Woo Kim, Hyoun Joong Yun, Sae Kil Chai, Ji Young Lee, Byeong Chun Park, Se Chang
Copyright Year	2015
Abstract	The increasing prevalence of antibiotic-resistant Shigella sp. emphasizes that alternatives to conventional antibiotics are needed. Siphoviridae bacteriophage (phage), pSf-2, infecting S. flexneri ATCC® 12022 was isolated from Geolpocheon stream in Korea. Morphological analysis by transmission electron microscopy revealed that pSf-2 has a head of about 57 ± 4 nm in diameter with a long tail of 136 ± 3 nm in length and 15 ± 2 nm in width. One-step growth analysis revealed that pSf-2 has latent period of 30 min and burst size of 16 PFU/infected cell. The DNA genome of pSf-2 is composed of 50,109 bp with a G+C content of 45.44 %. The genome encodes 83 putative ORFs, 19 putative promoters, and 23 transcriptional terminator regions. Genome sequence analysis of pSf-2 and comparative analysis with the homologous T1-like Shigella phages, Shfl1 and pSf-1, revealed that pSf-2 is a novel T1-like Shigella phage. These results showed that pSf-2 might have a high potential as a biocontrol agent to control shigellosis. Also, the genomic information may lead to further understanding of phage biodiversity, especially T1-like phages.
Starting Page	235
Ending Page	241
Page Count	7
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	72
Issue Number	3
e-ISSN	14320991
Language	English
Publisher	Springer US
Publisher Date	2015-11-26
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Comparative genomic analysis of bacteriophage EP23 infecting Shigella sonnei and Escherichia coli

Phenotypic characterization and genomic analysis of the Shigella sonnei bacteriophage SP18

Comparative genomic analysis of bacteriophage EP23 infecting Shigella sonnei and Escherichia coli.

Detection and Analysis of CRISPRs of Shigella

Isolation of Extremely AT-Rich Genomic DNA and Analysis of Genes Encoding Carbohydrate-Degrading Enzymes from Orpinomyces sp. Strain PC-2

Rapid isolation of fungal genomic DNA suitable for long distance PCR

Isolation and Characterization of a Novel Bacteriophage φ4D Lytic Against Enterococcus faecalis Strains

Isolation, characterization, and genomic analysis of the novel T4-like bacteriophage ΦCJ20.

Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea

Isolation and Comparative Genomic Analysis of T1-Like Shigella Bacteriophage pSf-2

Similar Documents

Comparative genomic analysis of bacteriophage EP23 infecting Shigella sonnei and Escherichia coli

Phenotypic characterization and genomic analysis of the Shigella sonnei bacteriophage SP18

Comparative genomic analysis of bacteriophage EP23 infecting Shigella sonnei and Escherichia coli.

Detection and Analysis of CRISPRs of Shigella

Isolation of Extremely AT-Rich Genomic DNA and Analysis of Genes Encoding Carbohydrate-Degrading Enzymes from Orpinomyces sp. Strain PC-2

Rapid isolation of fungal genomic DNA suitable for long distance PCR

Isolation and Characterization of a Novel Bacteriophage φ4D Lytic Against Enterococcus faecalis Strains

Isolation, characterization, and genomic analysis of the novel T4-like bacteriophage ΦCJ20.

Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea

Isolation and Comparative Genomic Analysis of T1-Like Shigella Bacteriophage pSf-2