NDLI: Characterization of a Cryptic and Intriguing Low Molecular Weight Plasmid

Content Provider	Springer Nature Link
Author	Carneiro, Lilian C. Mendes, Paulo Vinicius C. Silva, Silvana P. Souza, Guilherme R. L. Bataus, Luiz Artur M.
Copyright Year	2015
Abstract	The complete nucleotide sequence of cryptic plasmid pVCM04 isolated from Salmonella enterica serovar Enteritidis was determined and analyzed. pVCM04 contains 3853 bp with 53.6 % GC content and has twelve ORFs with more than 50 amino acids. Five of these sequences showed homology with replication and mobilization proteins. ORF1 and ORF2 showed homology with replication proteins, while ORFs 3–5 showed homology with mobilization proteins. The pVCM04 possesses a region associated with the theta-type replication mechanism. BLASTn search analysis revealed unexpectedly no similarity with sequences deposited in GenBank. The nucleotide sequence of pVCM04 can be divided into two arms: the region between nucleotides 552–1774 (encoding RepA and RepB) and the region between nucleotides 1775–3853 (encoding MobA, MobB and MobC). Codon bias pattern is distinct between mobA and repA, so the program Modeltest was used to select the best evolutionary model to study these genes. The result of ModelTest (model GTR+G for mobA and model HKY+G for repA) suggests that these genes would be subject to different selective pressures. Considering the differences in the codon usage, the selection of two different evolutionary models, and the absence of plasmids with homology to pVCM04 in GenBank, we believe that pVCM04 is a chimeric molecule and represents a new plasmid lineage.
Starting Page	351
Ending Page	356
Page Count	6
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	72
Issue Number	3
e-ISSN	14320991
Language	English
Publisher	Springer US
Publisher Date	2015-12-15
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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