NDLI: Reference strand conformational analysis (RSCA) is a valuable tool in identifying MHC-DRB sequences in three species of Aotus monkeys

Content Provider	Springer Nature Link
Author	Baquero, Juan E. Miranda, Santiago Murillo, Oscar Mateus, Heidy Trujillo, Esperanza Suarez, Carlos Patarroyo, Manuel E. Parra López, Carlos
Copyright Year	2006
Abstract	The Aotus monkey has been of great value in the pre-clinical study of malaria vaccine candidates. Several components of this primate’s immune system have been studied and they display great similarity to their human counterparts. Cloning and sequencing studies have revealed extensive sequence polymorphisms in Aotus MHC-DRB with very high similarities to several human allelic lineages, grouping at least nine distinct MHC-DRB lineages. As the efficacy of peptide vaccines in this animal model may be strongly influenced by exon 2 MHC-DRB polymorphism, the availability of a reliable and rapid MHC-DRB typing method for three species of Aotus (Aotus nancymaae, Aotus vociferans and Aotus nigriceps) is necessary. Reference strand conformational analysis (RSCA) was used here for differentiating the distinctive Aotus MHC-DRB sequences’ mobility using five fluorescently labelled references proved to be very useful for resolving closely related sequences, establishing the number of sequences transcribed in a particular monkey and their identity. The RSCA method’s reliability in terms of identifying Aotus MHC-DRB sequences will facilitate evaluating individual responsiveness to vaccines and prompt studies associating susceptibility/resistance to infectious agents or auto-immune disease, for which Aotus monkeys may be considered to be an appropriate animal model.
Starting Page	590
Ending Page	597
Page Count	8
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	58
Issue Number	7
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-05-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aotus monkeys MHC-DRB Reference strand conformational analysis (RSCA) Fluorescently labelled reference (FLR) Animal model Cell Biology Allergology Immunology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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