NDLI: Variation in the number of expressed MHC genes in different cattle class I haplotypes

Content Provider	Springer Nature Link
Author	Ellis, S. A. Holmes, E. C. Staines, K. A. Smith, K. B. Stear, M. J. McKeever, D. J. MacHugh, N. D. Morrison, W. I.
Copyright Year	1999
Abstract	Analysis of cattle major histocompatibility complex (MHC) (BoLA) class I gene expression using serological and biochemical methods has demonstrated a high level of polymorphism. However, analysis of class I cDNA sequences has failed to produce conclusive evidence concerning the number and nature of expressed genes. Such information is essential for detailed studies of cattle immune responses, and to increase our understanding of the mechanisms of MHC evolution. In this study a selective breeding programme has been used to generate a number of MHC homozygous cattle expressing common serologically defined class I specificities. Detailed analysis of five class I haplotypes was carried out, with transcribed class I genes identified and characterized by cDNA cloning, sequence analysis, and transfection/expression studies. Surface expression of the gene products (on lymphocytes) was confirmed using monoclonal antibodies of defined BoLA specificity. Phylogenetic analysis of available transcribed cattle MHC class I sequences revealed complex evolutionary relationships including possible evidence for recombination. The study of individual haplotypes suggests that certain groupings of related sequences may correlate with loci, but overall it was not possible to define the origin of individual alleles using this approach. The most striking finding of this study is that none of the cattle class I genes is consistently expressed, and that in contrast to human, haplotypes differ from one another in both the number and composition of expressed classical class I genes.
Starting Page	319
Ending Page	328
Page Count	10
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	50
Issue Number	5-6
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-12-02
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Generation and maintenance of diversity in the cattle MHC class I region

Genomic location and characterisation of nonclassical MHC class I genes in cattle

Analysis of MHC class I genes across horse MHC haplotypes

Characterization of MHC class I and β2-microglobulin sequences in Atlantic cod reveals an unusually high number of expressed class I genes

The assembly and characterisation of two structurally distinct cattle MHC class I haplotypes point to the mechanisms driving diversity

Serological and molecular diversity in the cattle MHC class I region

Defining the Turkey MHC: identification of expressed class I- and class IIB-like genes independent of the MHC-B

Multiple divergent haplotypes express completely distinct sets of class I MHC genes in zebrafish

Mhc class I genes of swordtail fishes, Xiphophorus: variation in the number of loci and existence of ancient gene families

Variation in the number of expressed MHC genes in different cattle class I haplotypes

Similar Documents

Generation and maintenance of diversity in the cattle MHC class I region

Genomic location and characterisation of nonclassical MHC class I genes in cattle

Analysis of MHC class I genes across horse MHC haplotypes

Characterization of MHC class I and β2-microglobulin sequences in Atlantic cod reveals an unusually high number of expressed class I genes

The assembly and characterisation of two structurally distinct cattle MHC class I haplotypes point to the mechanisms driving diversity

Serological and molecular diversity in the cattle MHC class I region

Defining the Turkey MHC: identification of expressed class I- and class IIB-like genes independent of the MHC-B

Multiple divergent haplotypes express completely distinct sets of class I MHC genes in zebrafish

Mhc class I genes of swordtail fishes, Xiphophorus: variation in the number of loci and existence of ancient gene families

Variation in the number of expressed MHC genes in different cattle class I haplotypes