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| Content Provider | Springer Nature Link |
|---|---|
| Author | Fujiki, Kazuhiro Gerwick, Lena Bayne, Christopher J. Mitchell, Leslie Gauley, Julie Bols, Niels Dixon, Brian |
| Copyright Year | 2003 |
| Abstract | A full-length cDNA encoding CCAAT/enhancer-binding protein β (C/EBPβ) was cloned from rainbow trout by anchored PCR. The putative 291 amino acid protein has 53% and 32% identity to the zebrafish and Japanese flounder sequences, respectively, and 30–34% identity to tetrapod homologues. This clone contains most conserved C/EBPβ domains except the second transactivation domain just like the zebrafish homologue. Also similar to zebrafish, rainbow trout produces only shorter C/EBPβ isoforms (LAP and LIP) but not the longer isoform (LAP*). However, unlike the zebrafish and Japanese flounder homologues, trout C/EBPβ has the short open reading frame (uORF) upstream of the start codon for LAP but in an alternate reading frame, a feature of tetrapod C/EBPβ genes. In normal rainbow trout, C/EBPβ mRNA was detected in peripheral blood leukocytes, head kidney, posterior kidney, liver, spleen, gills, intestine and muscle. RT-PCR revealed that transcript levels of trout C/EBPβ are clearly higher in sodium alginate-induced peritoneal cells than in head kidney and peritoneal cells of saline-injected fish or head kidney cells of alginate-injected fish. Together with expression in immunologically important tissues, this indicates that C/EBPβ is likely to be involved in the immune response just as in mammals. Southern hybridization suggested that C/EBPβ is a single copy gene. There are no introns in this C/EBPβ gene, just like the mammalian homologues. These data suggest that we have obtained the trout ortholog of C/EBPβ. |
| Starting Page | 253 |
| Ending Page | 261 |
| Page Count | 9 |
| File Format | |
| ISSN | 00937711 |
| Journal | Immunogenetics |
| Volume Number | 55 |
| Issue Number | 4 |
| e-ISSN | 14321211 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2003-05-28 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | C/EBPβ NFIL-6 Rainbow trout Suppression subtractive hybridization Transcription factor |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Immunology |
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