NDLI: Genomic characterization of MHC class I genes of the horse

Content Provider	Springer Nature Link
Author	Tallmadge, Rebecca L. Lear, Teri L. Antczak, Douglas F.
Copyright Year	2005
Abstract	The availability of a contig of bacterial artificial chromosome (BAC) clones spanning the equine major histocompatibility complex (MHC) made possible a detailed analysis of horse MHC class I genes. Prior to this study, only a single horse MHC class I gene had been sequenced at the genomic level. Although many (∼60) MHC class I cDNA sequences had been determined and published, from this information, it was not possible to determine how many class I loci are expressed in horses or to assign individual sequences to allelic series. In this study, 15 MHC class I genes were identified in BAC subclones and fully sequenced. Because the BAC library donor horse had been bred for homozygosity at the MHC, these 15 genomic clones represent distinct MHC class I genes and pseudogenes and not alleles at a smaller number of loci. For five of the genes, cDNA sequences from these loci had previously been identified. Two additional expressed class I genes were discovered, bringing the known total of different equine MHC class I genes (loci) expressed as mRNA to seven. Expression of all seven loci was detected by reverse transcriptase–polymerase chain reaction in adult, fetal, and placental tissues. The remaining eight genes were designated as pseudogenes. This work resulted in moderate expansion of the horse MHC BAC contig length, and the remaining gap was shortened. The information contained in these equine MHC class I sequences will permit comparison of MHC class I genes expressed across different horse MHC haplotypes and between horses and other mammalian species.
Starting Page	763
Ending Page	774
Page Count	12
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	57
Issue Number	10
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-10-12
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Horse MHC class I gene Genomic sequence Noncoding regions Cell Biology Allergology Immunology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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