NDLI: HLA-E, HLA-F, and HLA-G polymorphism: genomic sequence defines haplotype structure and variation spanning the nonclassical class I genes

Content Provider	Springer Nature Link
Author	Pyo, Chul Woo Williams, Luke M. Moore, Yuki Hyodo, Hirobu Li, Shuying Sue Zhao, Lue Ping Sageshima, riko Ishitani, Akiko Geraghty, Daniel E.
Copyright Year	2006
Abstract	Despite several studies that defined the polymorphism of the nonclassical human leukocyte antigen-E (HLA-E), HLA-F, and HLA-G genes, most polymorphisms thus far examined in correlative studies were derived from the coding sequences of these genes. In addition, some discrepancies and ambiguities in the available data have persisted in current databases. To expand the data available and to resolve some of the discrepant data, we have defined protocols that allow for the amplification of 6 to 7 kb of contiguous genomic sequence for each gene, including all of the coding and intron sequences, approximately 2 kb of 5' flanking promoter sequence, and 1 kb of 3' flanking sequence. Using long-range polymerase chain reaction (PCR) protocols, generating either one or two PCR products depending on the locus, amplified genomic DNA was directly sequenced to completion using a set of about 30 primers over each locus to yield contiguous sequence data from both strands. Using this approach, we sequenced 33 genomic DNAs, from Asian, African American, and Caucasian samples. The results of this analysis confirmed several previously reported coding sequence variants, identified several new allelic variants, and also defined extensive variation in intron and flanking sequences. It was possible to construct haplotype maps and to identify tagging single nucleotide polymorphisms that can be used to detect the composite variation spanning all three genes.
Starting Page	241
Ending Page	251
Page Count	11
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	58
Issue Number	4
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-03-29
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	MHC Nonclassical class I Genomics SNPs Alleles Allergology Cell Biology Gene Function Human Genetics Immunology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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HLA-E, HLA-F, and HLA-G polymorphism: genomic sequence defines haplotype structure and variation spanning the nonclassical class I genes