NDLI: Comparison of 16S rRNA and 16S rDNA T-RFLP Approaches to Study Bacterial Communities in Soil Microcosms Treated with Chromate as Perturbing Agent

Content Provider	Springer Nature Link
Author	Mengoni, Alessio Tatti, Enrico Decorosi, Francesca Viti, Carlo Bazzicalupo, Marco Giovannetti, Luciana
Copyright Year	2005
Abstract	Transcripts of ribosomal RNA have been used for assessing the structure and dynamics of active bacterial populations; however, it remains unclear whether the information provided by community profiling derived from RNA is different from that derived from DNA, particularly when a selective pressure is applied on the bacterial community.In the present work, terminal-restriction fragment length polymorphism (T-RFLP) community profiles based on DNA and RNA extracted from soil microcosms treated with a toxic concentration of chromate were compared.Microcosms of a nonpolluted agricultural soil and of a heavy-metal-rich soil (serpentine) were treated with chromate and DNA and RNA were extracted. T-RFLP analysis was performed on amplified and retro-amplified 16SrRNA gene sequences, and band profiles obtained from samples of DNA and of RNA were compared. Some of the T-RFLP bands, identified as peculiar peaks in the profiles, were cloned and sequenced for taxonomic interpretation.Results indicated that: (1) community profiles derived from RNA and DNA were partly overlapping; (2) there was a strong correlation between the dynamics shown by RNA- and DNA-based T-RFLP profiles; (3) chromate addition exerted a clear effect on both agricultural and serpentine soil bacterial communities, either at the DNA and at the RNA level; however, the profiles derived from RNA showed sharper differences between treated and control samples than that of DNA-based profiles.
Starting Page	375
Ending Page	384
Page Count	10
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	50
Issue Number	3
e-ISSN	1432184X
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-11-03
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Ecology Microbiology Geoecology/Natural Processes Nature Conservation
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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