NDLI: Comparison of Bacterial Communities in New England Sphagnum Bogs Using Terminal Restriction Fragment Length Polymorphism (T-RFLP)

Content Provider	Springer Nature Link
Author	Morales, Sergio E. Mouser, Paula J. Ward, Naomi Hudman, Stephen P. Gotelli, Nicholas J. Ross, Donald S. Lewis, Thomas A.
Copyright Year	2006
Abstract	Wetlands are major sources of carbon dioxide, methane, and other greenhouse gases released during microbial degradation. Despite the fact that decomposition is mainly driven by bacteria and fungi, little is known about the taxonomic diversity of bacterial communities in wetlands, particularly Sphagnum bogs. To explore bacterial community composition, 24 bogs in Vermont and Massachusetts were censused for bacterial diversity at the surface (oxic) and 1 m (anoxic) regions. Bacterial diversity was characterized by a terminal restriction fragment length (T-RFLP) fingerprinting technique and a cloning strategy that targeted the 16S rRNA gene. T-RFLP analysis revealed a high level of diversity, and a canonical correspondence analysis demonstrated marked similarity among bogs, but consistent differences between surface and subsurface assemblages. 16S rDNA sequences derived from one of the sites showed high numbers of clones belonging to the Deltaproteobacteria group. Several other phyla were represented, as well as two Candidate Division-level taxonomic groups. These data suggest that bog microbial communities are complex, possibly stratified, and similar among multiple sites.
Starting Page	34
Ending Page	44
Page Count	11
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	52
Issue Number	1
e-ISSN	1432184X
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-05-31
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Nature Conservation Geoecology/Natural Processes Microbiology Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

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