NDLI: Molecular Evolution of Miraculin-Like Proteins in Soybean Kunitz Super-Family

Content Provider	Springer Nature Link
Author	Selvakumar, Purushotham Gahloth, Deepankar Tomar, Prabhat Pratap Singh Sharma, Nidhi Sharma, Ashwani Kumar
Copyright Year	2012
Abstract	Miraculin-like proteins (MLPs) belong to soybean Kunitz super-family and have been characterized from many plant families like Rutaceae, Solanaceae, Rubiaceae, etc. Many of them possess trypsin inhibitory activity and are involved in plant defense. MLPs exhibit significant sequence identity (~30–95%) to native miraculin protein, also belonging to Kunitz super-family compared with a typical Kunitz family member (~30%). The sequence and structure–function comparison of MLPs with that of a classical Kunitz inhibitor have demonstrated that MLPs have evolved to form a distinct group within Kunitz super-family. Sequence analysis of new genes along with available MLP sequences in the literature revealed three major groups for these proteins. A significant feature of Rutaceae MLP type 2 sequences is the presence of phosphorylation motif. Subtle changes are seen in putative reactive loop residues among different MLPs suggesting altered specificities to specific proteases. In phylogenetic analysis, Rutaceae MLP type 1 and type 2 proteins clustered together on separate branches, whereas native miraculin along with other MLPs formed distinct clusters. Site-specific positive Darwinian selection was observed at many sites in both the groups of Rutaceae MLP sequences with most of the residues undergoing positive selection located in loop regions. The results demonstrate the sequence and thereby the structure–function divergence of MLPs as a distinct group within soybean Kunitz super-family due to biotic and abiotic stresses of local environment.
Starting Page	369
Ending Page	379
Page Count	11
File Format	PDF
ISSN	00222844
Journal	Journal of Molecular Evolution
Volume Number	73
Issue Number	5-6
e-ISSN	14321432
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-01-25
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rutaceae Kunitz super-family Miraculin-like proteins Sequence analysis Evolutionary relationships Positive selection Plant Sciences Cell Biology Microbiology Animal Genetics and Genomics Evolutionary Biology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Ecology, Evolution, Behavior and Systematics

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