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European Food Research and Technology : Volume 243, Issue 3, March 2017

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European Food Research and Technology : Volume 243, Issue 1, January 2017

European Food Research and Technology : Volume 242

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Development and evaluation of a dual priming oligonucleotide system-based multiplex PCR assay for simultaneous detection of six foodborne pathogens

Content Provider	Springer Nature Link
Author	Xu, Yi Gang Sun, Bo Zhao, Hai Yuan Liu, Zhong Mei Jiang, Yan Ping Wang, Li Qiao, Xin Yuan Li, Yi Jing Tang, Li Jie
Copyright Year	2016
Abstract	In this study, a multiplex PCR assay with dual priming oligonucleotide system (DPO system-based mPCR) was developed for the simultaneous detection of Salmonella spp., Listeria (L.) monocytogenes, Shigella spp., Staphylococcus (S.) aureus, Campylobacter (C.) jejuni and Yersinia (Y.) enterocolitica from food. Pathogen-specific DPO systems were designed targeting Salmonella spp. fimY gene, L. monocytogenes iap gene, Shigella spp. ipaH gene, S. aureus 442 gene, C. jejuni gyrA gene and Y. enterocolitica 16 s–23 s rRNA gene, respectively. Our optimized DPO system-based mPCR assay allowed a wide range of annealing temperature from 48 to 68 °C to efficiently amplify multi-genes followed by a nearly identical pattern with an analytical detection limit of 102–103 CFU/mL (g) for the simultaneous detection of the six target bacteria in pure cultures or artificially contaminated food matrixes. Applying the DPO system-based mPCR assay to 238 target and 83 nontarget bacterial strains revealed that only target bacterial strains were positive in this assay, indicating a high specificity. Moreover, the DPO system-based mPCR assay showed a potential diagnostic capability evaluated by testing 2419 samples collected from food, clinical and environmental sources. The DPO system-based mPCR assay may provide a useful tool for the detection of these six foodborne pathogens in laboratory diagnosis.
Starting Page	555
Ending Page	563
Page Count	9
File Format	PDF
ISSN	14382377
Journal	European Food Research and Technology
Volume Number	243
Issue Number	4
e-ISSN	14382385
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-09-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Foodborne pathogens Dual priming oligonucleotide (DPO) system Multiplex PCR assay Food Science Analytical Chemistry Biotechnology Agriculture Forestry
Content Type	Text
Resource Type	Article
Subject	Industrial and Manufacturing Engineering Chemistry Biochemistry Food Science Biotechnology

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