NDLI: Five pentaplex real-time PCR systems for the efficient determination of 20 genetically modified maize traits in food

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Five pentaplex real-time PCR systems for the efficient determination of 20 genetically modified maize traits in food

Content Provider	Springer Nature Link
Author	Köppel, René Ganeshan, Arthika Velsen, Franziska Bucher, Thomas
Copyright Year	2016
Abstract	According to the EU and Swiss food legislation, only authorised traits of transgene plants are allowed to be imported and sold to the consumer. In order to control imports of maize products from retailers, efficient and reliable methods for the detection and quantification are a prerequisite. Actually, in the EU, more than 25 gm Maize traits were authorised and are allowed to be sold. To ensure correct labelling and exclude unauthorised gm traits from sale, five multiplex real-time polymerase chain reaction systems were developed and validated. These 20 systems for the most prevalent maize gm traits allow relative multiplex quantification and/or delta–delta Ct method quantification in routine in a reliable, cost- and time-efficient way.
Starting Page	215
Ending Page	225
Page Count	11
File Format	PDF
ISSN	14382377
Journal	European Food Research and Technology
Volume Number	243
Issue Number	2
e-ISSN	14382385
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-06-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	gm Maize Multiplex Real-time quantitative PCR Validation Food Science Analytical Chemistry Biotechnology Agriculture Forestry
Content Type	Text
Resource Type	Article
Subject	Industrial and Manufacturing Engineering Chemistry Biochemistry Food Science Biotechnology

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