NDLI: Measurement of Giardia lamblia adhesion force using an integrated microfluidic assay

Content Provider	Springer Nature Link
Author	Lu, Ling Zheng, Guo Xia Yang, Yu Suo Feng, Cheng Yu Liu, Fang Fang Wang, Yun Hua
Copyright Year	2016
Abstract	The mechanisms how Giardias attach to the intestinal epithelium remain unclear. None of the methods currently being used to measure the attachment force could provide a continuous nutrition supply and a micro-aerobic atmosphere to the Giardia. Besides, they are all labor-intensive. In the present research, a microfluidic method based on electric circuit analogy was developed. The input fluid flowed through the inlet channel with different lengths and was distributed in four assay chambers. Shear force gradients were generated in chambers, too. This allowed an easy control of fluids and the shear forces. Most importantly, the shear stress large enough to detach Giardia could be generated in laminar flow regime. Moreover, analysis could be accomplished in one single test. By applying inlet flow rates of 30, 60, and 120 μL ml–1, shear force gradients ranging from 19.47 to 60.50 Pa were generated. The adhesion forces of trophozoites were analyzed and the EC50 of the force that caused 50% trophozoites detachment was calculated as 36.60 Pa. This paper presents a novel method for measurement of Giardia adhesion force. Graphical Abstract Measurement of Giardia adhesion force. Various of flow rates were applied to generate different shear forces and Giardia trophozoites remaining attached were counted (a-c). The percentages of attachment vs shear stress were plotted and the EC50 of adhesion force was calculated (d)
Starting Page	1451
Ending Page	1459
Page Count	9
File Format	PDF
ISSN	16182642
Journal	Analytical and Bioanalytical Chemistry
Volume Number	409
Issue Number	5
e-ISSN	16182650
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-11-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Giardia lamblia Microfluidic Attachment Shear force Analytical Chemistry Biochemistry Laboratory Medicine Characterization and Evaluation of Materials Food Science Monitoring/Environmental Analysis
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Measurement uncertainty challenge

Novel concept of washing for microfluidic paper-based analytical devices based on capillary force of paper substrates

Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry

Isotope amount ratio measurement challenge

Analytical electrochemiluminescence

Solution to isotope amount ratio measurement challenge

Reconstruction of undergraduate analytical chemistry laboratory course

N-Glycan profile analysis of transferrin using a microfluidic compact disc and MALDI-MS

Hollandaise challenge

Measurement of Giardia lamblia adhesion force using an integrated microfluidic assay

Similar Documents

Measurement uncertainty challenge

Novel concept of washing for microfluidic paper-based analytical devices based on capillary force of paper substrates

Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry

Isotope amount ratio measurement challenge

Analytical electrochemiluminescence

Solution to isotope amount ratio measurement challenge

Reconstruction of undergraduate analytical chemistry laboratory course

N-Glycan profile analysis of transferrin using a microfluidic compact disc and MALDI-MS

Hollandaise challenge

Measurement of Giardia lamblia adhesion force using an integrated microfluidic assay