NDLI: Ovine synovial membrane-derived mesenchymal progenitor cells retain the phenotype of the original tissue that was exposed to in-vivo inflammation: evidence for a suppressed chondrogenic differentiation potential of the cells

Content Provider	Springer Nature Link
Author	Ando, Wataru Heard, Bryan J. Chung, May Nakamura, rimasa Frank, Cyril B. Hart, David A.
Copyright Year	2012
Abstract	The purpose of this study was to characterize the effect of post-surgery joint inflammation on the chondrogenic differentiation capacity of mesenchymal progenitor cells (MPCs) derived from the synovial membrane (SM).Six Suffolk-cross sheep were subjected to experimental anterior cruciate ligament (ACL) core surgeries. After they were killed 2 weeks after surgery, the volume of synovial fluid in the knees was measured and SM was collected for mRNA extraction and cell isolation. Cells were propagated and used for lineage-specific differentiation assays using cell pellet cultures and mRNA extraction. Chondrogenic differentiation assays in the presence of exogenous interleukin-1β (IL-1β) were also performed.The volume of synovial fluid from the operated knees was significantly greater than from the contralateral knees. Quantitative RT-PCR revealed that mRNA levels for IL-1β and matrix metalloproteinases-3 and -13 in SM from the operated knees were significantly higher than those from the contralateral knees. The size of MPC pellets from operated knees (opMPC) cultured in chondrogenic medium were significantly smaller than the corresponding pellets generated with MPCs from contralateral knees (conMPC). Addition of 1–100 ng/ml IL-1β significantly suppressed the resultant size of chondrogenic cell pellets from normal ovine SM-MPC.From these results, we conclude that cells from SM exposed to post-surgical inflammation are compromised by the inflammatory environment and that IL-1β can inhibit the latent chondrogenic potential of normal MPCs. This suggests that if MPCs from injured joints do contribute to cartilage repair, their endogenous repair potential may become compromised by such post-injury joint inflammation.
Starting Page	599
Ending Page	608
Page Count	10
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	61
Issue Number	6
e-ISSN	1420908X
Language	English
Publisher	SP Birkhäuser Verlag Basel
Publisher Date	2012-03-04
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chondrogenesis Inflammation Interleukin (IL) Matrix metalloproteinases (MMP) Mesenchymal progenitor cells (MPCs) Neurology Immunology Pharmacology/Toxicology Dermatology Rheumatology Allergology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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