NDLI: Construction of a recombinant adenovirus vector expressing IL-18BP/IL-4 fusion gene and the anti-inflammatory effect induced by this gene on lipopolysaccharide-stimulated synovial fibroblasts

Content Provider	Springer Nature Link
Author	Yao, Hang Ping Qian, Yun Shao, Xue Ting Xu, Zhe Rong Cheng, Lin Fang Feng, Lei Wu, Nan Ping Yang, Yun Mei
Copyright Year	2009
Abstract	To construct a recombinant adenovirus vector for expressing the IL-18 binding protein (IL-18BP)/IL-4 fusion gene and confirm the anti-inflammatory effect of this gene.The recombinant virus expressing IL-18BP/IL-4 fusion protein (AD-IL-18BP/IL-4) was constructed. AD-IL-18BP/IL-4 was used to infect synovial fibroblasts (SF). ELISA and Western blot analysis were used to determine the expressions of the proteins IL-4 and IL-18BP. To investigate the protective effects of this vector on rheumatoid arthritis, SF were infected with AD-IL-18BP/IL-4 and stimulated by LPS (1 μg/ml) 4 h later. The expression levels of TNF-α, IL-6, IL-8, and IL-18 in the culture supernatant were detected by ELISA and production of PGE2 and NO was estimated. The protein expression of COX-2, iNOS, and NF-κB p50 in treated SF was analyzed by Western blot.AD-IL-18BP/IL-4 can effectively express the IL-18BP/IL-4 fusion protein. The expressions of TNF-α, IL-6, IL-8, and IL-18 were significantly inhibited in LPS-stimulated SF after treatment with AD-IL-18BP/IL-4. The production of PGE2 and NO was significantly decreased. Moreover, NF-κB p50, COX-2, and iNOS levels in SF were markedly suppressed by AD-IL-18BP/IL-4.AD-IL-18BP/IL-4 can suppress the production and expression of inflammatory cytokines such as COX-2, iNOS, and NF-κB in LPS-stimulated SF.
Starting Page	97
Ending Page	104
Page Count	8
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	59
Issue Number	2
e-ISSN	1420908X
Language	English
Publisher	SP Birkhäuser Verlag Basel
Publisher Date	2009-11-22
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Interleukin-18BP Interleukin-4 Fusion gene Synovial fibroblasts Anti-inflammation Neurology Dermatology Allergology Rheumatology Pharmacology/Toxicology Immunology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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