NDLI: Detection of a CD4+CD8−CD3− cell subpopulation during the differentiation of cord blood CD34+ cells into T cells in vitro

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Detection of a CD4+CD8−CD3− cell subpopulation during the differentiation of cord blood CD34+ cells into T cells in vitro

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Archivum Immunologiae et Therapiae Experimentalis : Volume 57, Issue 2, April 2009

Archivum Immunologiae et Therapiae Experimentalis : Volume 57, Issue 1, February 2009

Archivum Immunologiae et Therapiae Experimentalis : Volume 56

Archivum Immunologiae et Therapiae Experimentalis : Volume 55

Archivum Immunologiae et Therapiae Experimentalis : Volume 54

Detection of a CD4+CD8−CD3− cell subpopulation during the differentiation of cord blood CD34+ cells into T cells in vitro

Content Provider	Springer Nature Link
Author	Jin, Jian Gang Bai, Bao Jun Yao, Zhi Juan Wu, Ren Na Feng, Kai Hu, Jiang Wei Hu, Liang Ding Jiang, Min Liao, Lianming Chen, Hu
Copyright Year	2009
Abstract	Umbilical cord blood contains relatively abundant primitive CD34+ hematopoietic progenitor cells which can differentiate into T lymphocytes ex vivo In this study, thymic stromal cells (TSCs) were isolated from aborted fetuses and a monolayer culture system was established. Highly-purified CD34+ cells from umbilical cord blood were cultured on the TSCs after limiting-dilution. The cells were then harvested and evaluated for CD4, CD8, and CD3 expression at different time points. CD4+CD8−CD3− lymphoid progenitor cells that could differentiate into mature T lymphocytes were observed after 15 days when a cocktail of cytokines, including Flt-3 ligand, stem cell factor, interleukin (IL)-12, and IL-2, was added.These results thus show that CD4+CD8−CD3− cells can be derived from CD34+ cells in vitro when cultured on TSCs.We showed that CD4+CD8−CD3− cells can be derived from highly purified CD34+ cells on TSCs during T-cell lymphopoiesis in vitro.
Starting Page	213
Ending Page	219
Page Count	7
File Format	PDF
ISSN	0004069X
Journal	Archivum Immunologiae et Therapiae Experimentalis
Volume Number	57
Issue Number	3
e-ISSN	16614917
Language	English
Publisher	SP Birkhäuser Verlag Basel
Publisher Date	2009-05-29
Publisher Place	Basel
Access Restriction	Subscribed
Subject Keyword	thymus cell differentiation stromal cells T cells Pharmacology/Toxicology Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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