NDLI: Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

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Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

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Archivum Immunologiae et Therapiae Experimentalis : Volume 57, Issue 2, April 2009

Archivum Immunologiae et Therapiae Experimentalis : Volume 57, Issue 1, February 2009

Archivum Immunologiae et Therapiae Experimentalis : Volume 56

Archivum Immunologiae et Therapiae Experimentalis : Volume 55

Archivum Immunologiae et Therapiae Experimentalis : Volume 54

Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

Content Provider	Springer Nature Link
Author	Çanakçi, Cenk Fatih Çanakçi, Varol Tatar, Abdulgani Eltas, Abubekir Sezer, Ufuk Çiçek, Yasin Oztas, Sitki
Copyright Year	2009
Abstract	Oxidative stress may contribute to the pathogenesis of periodontitis. However, the detailed molecular mechanism remains unclear. Both 8-hydroxydeoxyguanosine (8-OHdG) and mitochondrial DNA (mtDNA) deletion have been reported as early oxidative DNA damage markers. In this study, 8-OHdG levels in saliva and mtDNA deletions in gingival tissue of patients with chronic periodontitis (CP) were evaluated.Gingival tissue and whole saliva samples were collected from 32 patients with CP and 32 healthy control subjects. To determine the clinical condition of each subject, the plaque index, gingival index, clinical attachment level (CAL), and probing depth (PD) were measured. Using the ELISA and polymerase chain reaction methods, the salivary 8-OHdG levels and the 7.4-kbp and 5-kbp mtDNA deletions were examined.The 5-kbp mtDNA deletion was detected in 20 of the 32 periodontitis patients (62.5%), but was not detected in the healthy controls. The mean value of 8-OHdG in the saliva of the periodontitis patients with deleted mtDNA was significantly higher than in the patients with non-deleted mtDNA (p<0.01). Also, significant correlation was found between the occurrence of the 5-kbp mtDNA deletion and salivary 8-OHdG levels (p<0.01). Similar correlations were detected between salivary 8-OHdG levels and age, PD, and CAL (p<0.01, p<0.05).Increased oxidative stress may lead to premature oxidative DNA damage in the gingival tissue of periodontitis patients and the salivary 8-OHdG level may signify premature oxidative mtDNA damage in diseased gingival tissue.
Starting Page	205
Ending Page	211
Page Count	7
File Format	PDF
ISSN	0004069X
Journal	Archivum Immunologiae et Therapiae Experimentalis
Volume Number	57
Issue Number	3
e-ISSN	16614917
Language	English
Publisher	SP Birkhäuser Verlag Basel
Publisher Date	2009-05-29
Publisher Place	Basel
Access Restriction	Subscribed
Subject Keyword	periodontitis gingiva saliva 8-hydroxyguanosine mitochondrial DNA deletion oxidative DNA damage Pharmacology/Toxicology Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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