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Prepubertal testis development relies on retinoic acid but not rexinoid receptors in Sertoli cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Vernet, Nadège Dennefeld, Christine Guillou, Florian Chambon, Pierre Ghyselinck, Norbert B. Mark, Manuel |
| Copyright Year | 2006 |
| Abstract | Sertoli cells (SC) are instrumental to stem spermatogonia differentiation, a process that critically depends on retinoic acid (RA). We show here that selective ablation of RA receptor alpha (RARalpha) gene in mouse SC, singly (Rara(Ser-/-) mutation) or in combination with RARbeta and RARgamma genes (Rara/b/g(Ser-/-) mutation), abolishes cyclical gene expression in these cells. It additionally induces testis degeneration and delays spermatogonial expression of Stra8, two hallmarks of RA deficiency. As identical defects are generated upon inactivation of RARalpha in the whole organism, our data demonstrate that all the functions exerted by RARalpha in male reproduction are Sertoli cell-autonomous. They further indicate that RARalpha is a master regulator of the cyclical activity of SC and controls paracrine pathways required for spermatogonia differentiation and germ cell survival. Most importantly, we show that the ablation of all RXR (alpha, beta and gamma isotypes) in SC does not recapitulate the phenotype generated upon ablation of all three RARs, thereby providing the first evidence that RARs exert functions in vivo independently of RXRs. |
| Starting Page | 76 |
| Ending Page | 79 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://emboj.embopress.org/content/embojnl/25/24/5816.full.pdf |
| PubMed reference number | 17124491v1 |
| Volume Number | 25 |
| Issue Number | 24 |
| Journal | The EMBO journal |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cell Survival Gene Expression Germ Cells Immunoglobulin Isotypes Interferon Type II Mutation Prepuberty Refractory anemia with ringed sideroblasts Retinoic Acid Receptor Alpha, human Retinoid X Receptors Spermatogonia Structure of sertoli cell Tretinoin male gonad development physical hard work |
| Content Type | Text |
| Resource Type | Article |
