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Incidência de fungos do gênero Aspergillus seção Circumdati e Nigri em grãos de café cultivados em Minas Gerais e o efeito in vitro de fatores abióticos no crescimento e na biossíntese de ocratoxina A
| Content Provider | Semantic Scholar |
|---|---|
| Author | Oliveira, Gislaine |
| Copyright Year | 2016 |
| Abstract | Ochratoxin A (OTA) is considered the main mycotoxin associated with coffee, produced mainly by fungi of the genus Aspergillus, sections Circumdati and Nigri. This study was conducted to evaluate the fungi incidence of the genus Aspergillus, section Circumdati and Nigri, in coffee beans grown in two producing regions of Minas Gerais. It was analyzed 14 samples of Arabica coffee beans (Coffea arabica L.), seven of them being of the South of Minas and seven in the Cerrado region of Minas. The isolation of the fungi Aspergillus, Section Circumdati and Nigri, was carried out by plating technique straight amid dichloran rose bengal chloramphenicol (DRBC). The identification was based on morphological characteristics. The OTA quantification of the isolates was performed by HPLC. Ninety (90) fungi of the genus Aspergillus Section Nigri and Circumdati were isolated and identified, of which 82% were owned by section Nigri and 18% belonging to Section Circumdati. The percentage of coffee beans contamination grown in the South of Minas was 65.5%. Samples of the Cerrado Mineiro have not elevated fungal contamination, and the percentage of coffee beans contamination in this region was 34.5%, all in Section Nigri. The species that were evaluated, the most often found in the two regions was A. niger. In this study, 43.24% of the isolates belonging to Section Nigri were OTA producers and, of these, 84% were A. niger and 16%, A. carbonarius. The isolates that were belonging to the genus Aspergillus, Section Circumdati, 81.25% were producers of OTA, of wich 69% A. ochraceus and 31% A.ostianus. Although in this study, isolated from A. niger have been found most frequently, the average concentration of OTA produced by these fungi (0.72 μg OTA/g) was much lower compared with the average concentration of OTA produced by A. ochraceus (31.2 μg OTA/g). The presence of fungi toxigenic not necessarily indicate the presence of OTA, but indicates a potential risk. The knowledge of toxigenic species is essential for adopting measures to control and guarantee a safe product. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.sbicafe.ufv.br/bitstream/handle/123456789/9108/Tese_Gislaine%20Oliveira.pdf?isAllowed=y&sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
