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Protein engineering of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Liu, Yuan-Ting Chang, C. Shie, Wen-Shiang Wu, Tung-Kung |
| Copyright Year | 2012 |
| Abstract | A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7(T384Y/Q450H/V454I), produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae. |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/ol302341h |
| Alternate Webpage(s) | https://ir.nctu.edu.tw/bitstream/11536/20423/1/000309951500015.pdf |
| Alternate Webpage(s) | http://www.qmclab.com/labwiki/files/literature/terpene/triterpene/Oxidosqualene%20Cyclase/mutation%20of%20ERG7/(2012%20Org.%20Lett.%20Tung-Kung%20Wu%20)+ERG7%20(%20T384Y%20+%20Q450H%20+%20V454%20mutant).pdf |
| PubMed reference number | 23043506 |
| Alternate Webpage(s) | https://doi.org/10.1021/ol302341h |
| Journal | Medline |
| Volume Number | 14 |
| Issue Number | 20 |
| Journal | Organic letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |