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Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Suárez, Cecilia Díaz-Torga, G. Vela, Jorge Espino Mladovan, Alejandro Gustavo Baldi, Alberto Becú-Villalobos, Damasia |
| Copyright Year | 2003 |
| Abstract | We studied the effects of ANG II on extracellular signal-regulated kinase (ERK)1/2 phosphorylation in rat pituitary cells. ANG II increased ERK phosphorylation in a time- and concentration-dependent way. Maximum effect was obtained at 5 min at a concentration of 10-100 nM. The effect of 100 nM ANG II was blocked by the AT1 antagonist DUP-753, by the phospholipase C (PLC) inhibitor U-73122, and by the MAPK kinase (MEK) antagonist PD-98059. The ANG II-induced increase in phosphorylated (p)ERK was insensitive to pertussis toxin blockade and PKC depletion or inhibition. The effect was also abrogated by chelating intracellular calcium with BAPTA-AM or TMB-8 by depleting intracellular calcium stores with a 30-min pretreatment with EGTA and by pretreatment with herbimycin A and PP1, two c-Src tyrosine kinase inhibitors. It was attenuated by AG-1478, an inhibitor of epidermal growth factor receptor (EGFR) activation. Therefore, in the rat pituitary, the increase of pERK is a Gq- and PLC-dependent process, which involves an increase in intracellular calcium and activation of a c-Src tyrosine kinase, transactivation of the EGFR, and the activation of MEK. Finally, the response of ERK activation by ANG II is altered in hyperplastic pituitary cells, in which calcium mobilization evoked by ANG II is also modified. |
| Starting Page | 36 |
| Ending Page | 43 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ajpendo.physiology.org/content/ajpendo/285/3/e645.full.pdf |
| PubMed reference number | 12759218v1 |
| Volume Number | 285 |
| Issue Number | 3 |
| Journal | American journal of physiology. Endocrinology and metabolism |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Angiotensin II Angiotensins Chelating Activity DuP-753 EGFR protein, human Egtazic Acid Growth Factor Receptors Hyperplasia MAPK-ERK Kinases PD 98059 Pertussis Toxin Pertussis Vaccine Phospholipase C Pituitary Hormones, Anterior Protein Kinase C Protein Tyrosine Kinase TMB 8 Trans-Activation, Genetic U 73122 herbimycin release of sequestered calcium ion into cytoplasm tyrphostin AG 1478 |
| Content Type | Text |
| Resource Type | Article |
