NDLI: Activation of Akt/protein kinase B after stimulation with angiotensin II in vascular smooth muscle cells.

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Activation of Akt/protein kinase B after stimulation with angiotensin II in vascular smooth muscle cells.

Content Provider	Semantic Scholar
Author	Takahashi, Takiko Taniguchi, Takahiro Konishi, Hakuou Kikkawa, Ushio Ishikawa, Yuichi Yokoyama, Masayoshi
Copyright Year	1999
Abstract	Involvement of Akt/Protein kinase B (PKB), a serine/threonine kinase with a pleckstrin-homology domain, in angiotensin II (ANG II)-induced signal transduction was investigated in cultured vascular smooth muscle cells (VSMC). Stimulation of the cells with ANG II led to a marked increase in the kinase activity of Akt/PKB, which coincided with Ser-473 phosphorylation. ANG II-stimulated Akt/PKB activation was rapid, concentration dependent, and inhibited by the AT1-receptor antagonist CV-11974, but not by pertussis toxin. Akt/PKB activity was stimulated by the Ca2+ ionophore ionomycin, suggesting the possible involvement of Ca2+ in ANG II-stimulated Akt/PKB activation. However, blockade of Ca2+ mobilization by BAPTA-AM only partially inhibited ANG II-stimulated Akt/PKB activation. ANG II-stimulated Akt/PKB activation was inhibited by the tyrosine kinase inhibitors genistein and herbimycin A and by the phosphatidylinositol 3-kinase (PI3K) inhibitors wortmannin and LY-294002. These results indicate that ANG II stimulates Akt/PKB activity via AT1 receptors in VSMC and that the activities of tyrosine kinase and PI3K are required for this activation.
Starting Page	42
Ending Page	48
Page Count	7
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://ajpheart.physiology.org/content/ajpheart/276/6/H1927.full.pdf
PubMed reference number	10362672v1
Alternate Webpage(s)	https://doi.org/10.1152/ajpheart.1999.276.6.H1927
DOI	10.1152/ajpheart.1999.276.6.H1927
Journal	The American journal of physiology
Volume Number	276
Issue Number	6
Language	English
Access Restriction	Open
Subject Keyword	1-Phosphatidylinositol 3-Kinase Angiotensin II Angiotensins CV-11974 Genistein Homologous Gene Ionomycin Ionophores LY 294002 MARK2 gene Muscle Cells Muscle, Smooth, Vascular Myocytes, Smooth Muscle Pertussis Toxin Pleckstrin Homology Domains Protein Kinases Protein Tyrosine Kinase Protein-Serine-Threonine Kinases Proto-Oncogene Proteins c-akt Signal Transduction Smooth muscle (tissue) Threonine eIF-2 Kinase herbimycin kinase activity regulation of sequestering of calcium ion wortmannin
Content Type	Text
Resource Type	Article

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