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Angiotensin II increases Pax-2 expression in fetal kidney cells via the AT2 receptor.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Shao-Ling Moini, Babak Ingelfinger, Julie R. |
| Copyright Year | 2004 |
| Abstract | Although both the renin angiotensin system (RAS) and the paired homeobox 2 gene (Pax-2) seem critically important in renal organogenesis, whether and how they might interact has not been addressed. The present study asked whether a link between the RAS and Pax-2 exists in fetal renal cells, speculating that such an interaction, if present, might influence renal development. Embryonic kidney explants and embryonic renal cells (mouse late embryonic mesenchymal epithelial cells [MK4] and mouse early embryonic mesenchymal fibroblasts [MK3]) were used. Pax-2 protein and Pax-2 mRNA were detected by immunofluorescence, Western blot, reverse transcription-PCR, and real-time PCR. Angiotensin II (AngII) upregulated Pax-2 protein and Pax-2 mRNA expression via the AngII type 2 (AT(2)) receptor in MK4 but not in MK3 cells. The stimulatory effect of AngII on Pax-2 gene expression could be blocked by PD123319 (AT(2) inhibitor), AG 490 (a specific Janus kinase 2 inhibitor), and genistein (a tyrosine kinase inhibitor) but not by losartan (AT(1) inhibitor), SB203580 (specific p38 mitogen-activated protein kinase inhibitor), PD98059 (specific MEK inhibitor), SP600125 (JNK inhibitor), and diphenyleneiodonium chloride (an NADPH oxidase inhibitor). Moreover, embryonic kidney explants in culture confirmed that AngII upregulates Pax-2 gene expression via the AT(2) receptor. These studies demonstrate that the stimulatory effect of AngII on Pax-2 gene expression is mediated, at least in part, via the Janus kinase 2/signal transducers and activators of transcription signaling transduction pathway, suggesting that RAS and Pax-2 interactions may be important in renal development. |
| Starting Page | 4137 |
| Ending Page | 4150 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jasn.asnjournals.org/content/15/6/1452.full.pdf |
| PubMed reference number | 15153556v1 |
| Volume Number | 15 |
| Issue Number | 6 |
| Journal | Journal of the American Society of Nephrology : JASN |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Angiotensin II Angiotensins Gene Expression Genistein Immunofluorescence assay Janus kinase Kidney Diseases Losartan Mitogen-Activated Protein Kinases Mitogens NADPH Oxidase Organogenesis PD 98059 Protein Kinase Inhibitors Protein Tyrosine Kinase Protein-tyrosine kinase inhibitor Renal Tissue Renin-angiotensin system Reverse Transcription SB 203580 SP600125 Silver Transducers Western Blot diphenyleneiodium chloride |
| Content Type | Text |
| Resource Type | Article |
